首页 > 新书资源
新书资源(2009年10月)

Molecular biology and biotechnology / edited by John M. Walker and Ralph Rapley. — 5th ed. — Cambridge, UK : Royal Society of Chemistry, c2009. – (58.17/M718c/5th ed.)

Contents

    Contents
    
    Chapter 1 Basic Molecular Biology Techniques
    1.1 Enzymes Used in Molecular Biology
    1.2 Isolation and Separation of Nucleic Acids
    1.3 Electrophoresis of Nucleic Acids
    1.4 Restriction Mapping of DNA Fragments
    1.5 Nucleic Acid Analysis Methods
    1.6 Gene Probe Derivation
    1.7 Labelling DNA Gene Probe Molecules
    1.8 The Polymerase Chain Reaction References
    Chapter 2 Molecular Cloning and Protein Expression
    2.1 Introduction
    2.2 Host-related Issues
    2.3 Vectors
    2.4 Expression Systems
    2.5 Problems
    2.6 Fusion Proteins
    2.7 Other Hosts
    2.8 Cell-free Systems
    2.9 Conclusion
    References
    Chapter 3 Molecular Diagnostics
    3.1 Introduction
    3.2 Technologies
    3.3 The Infectious Disease Paradigm
    3.4 Genetics
    3.5 Hematology
    3.6 Oncology
    3.7 Pharmacogenomics
    3.8 Conclusion
    References
    Chapter 4 Molecular Microbial Diagnostics
    4.1 Introduction
    4.2 Classical Microbiological Diagnosis
    4.3 Sample Collection and Nucleic Acid Purification
    4.4 Nucleic Acid Amplification Techniques
    4.5 Other Techniques Used in Clinical Microbiology
    4.6 Selected Examples of Clinical Nucleic Acid-based Diagnosis
    4.7 Conclusion and Future Challenges
    References
    Chapter 5 Genes and Genomes
    5.1 Introduction
    5.2 Key DNA Technologies
    5.3 The Polymerase Chain Reaction (PCR)
    5.4 DNA Sequencing
    5.5 Genome Analysis
    5.6 Genome Projects Background
    5.7 Gene Discovery and Localisation
    5.8 Future Directions References
    Chapter 6 The Biotechnology and Molecular Biology of Yeast
    6.1 Introduction
    6.2 The Production of Heterologous Proteins by Yeast
    6.3 From Re-engineering Genomes to Constructing Novel Signal and Biochemical Pathways
    6.4 Yeast as a Paradigm of Eukaryotic Cellular Biology
    6.5 Future Prospects
    References
    Chapter 7 Metabolic Engineering
    7.1 Introduction
    7.2 Theoretical Approaches for Metabolic Networks
    7.3 Experimental Approaches for Metabolic Engineering
    7.4 Examples in Metabolic Engineering
    7.5 Omics Technologies Open New Perspectives for Metabolic Engineering
    7.6 Acknowledgement
    References
    Chapter 8 Bionanotechnology
    8.1 Introduction
    8.2 Semiconductor Quantum Dots
    8.3 Magnetic Nanoparticles
    8.4 Zerovalent Noble Metal Nanoparticles
    8.5 Making Nanoscale Structures Using Biotechnology
    8.6 Conclusions
    References
    Chapter 9 Molecular Engineering of Antibodies
    9.1 Introduction
    9.2 Antibodies as Therapeutics
    9.3 Antibody Structure and Function
    9.4 Chimeric Antibodies
    9.5 Antibody Humanization
    9.6 Antibodies from Diversity Libraries and Display Technologies
    9.7 Engineering Antibody Affinity
    9.8 Enhancing Antibody Potency
    9.9 Conclusion
    References
    Chapter 10 Plant Biotechnology
    10.1 Introduction
    10.2 Applications of Molecular Biology to Speed Up the Processes of Crop Improvement
    10.3 Transgenic Technologies
    10.4 Applications of Transgenic Technologies
    10.5 Engineering Crop Resistance to Herbicides
    10.6 Engineering Resistance to Pests And Diseases
    10.7 Manipulating Male Sterility
    10.8 Tolerance to Abiotic Stresses
    10.9 Manipulating Quality
    10.10 Production of Plant Polymers and Biodegradable Plastics
    10.11 Plants as Bioreactors: Biopharming and Neutraceuticals
    10.12 Plant Biotechnology in Forestry
    10.13 Intellectual Property
    10.14 Public Acceptance
    10.15 Future Prospects
    References
    Chapter 11 Biotechnology-based Drug Discovery
    11.1 Introduction to Drug Discovery
    11.2 New Biotechnologies for Drug Discovery
    11.3 Genomic Technologies for Drug Discovery
    11.4 Role of Proteomics in Drug Discovery
    11.5 Metabolomic and Metabonomic Technologies for Drug Discovery
    11.6 Role of Nanobiotechnology in Drug Discovery
    11.7 Role of Biomarkers in Drug Discovery
    11.8 Screening in Drug Discovery
    11.9 Target Validation Technologies
    11.10 Antisense for Drug Discovery
    11.11 RNAi for Drug Discovery
    11.12 Biochips and Microarrays for Drug Discovery
    11.13 Applications of Bioinformatics in Drug Discovery
    11.14 Role of Model Organisms in Drug Discovery
    11.15 Chemogenomic Approach to Drug Discovery
    11.16 Virtual Drug Development
    11.17 Role of Biotechnology in Lead Generation and Validation
    11.18 Conclusion
    References
    Chapter 12 Vaccines
    12.1 An Overview of Vaccines and Vaccination
    12.2 Types of Vaccines in Current Use
    12.3 The Need for New Vaccines
    12.4 New Approaches to Vaccine Development
    12.5 Adjuvants
    References
    Chapter 13 Tissue Engineering
    13.1 Introduction
    13.2 Cell Types
    13.3 Extracellular Matrix
    13.4 Tissue Engineering Concepts
    13.5 Conclusions
    References
    Chapter 14 Transgenesis
    14.1 Introduction
    14.2 Transgenesis by DNA Pronuclear Injection
    14.3 Gene Targeting by Homologous Recombination in Embryonic Stem Cells
    14.4 Conditional Gene Targeting
    14.5 Phenotypic Analysis of Genetically Modified Mice
    14.6 Ethical and Animal Welfare Considerations
    14.7 Conclusions
    14.8 Acknowledgements
    References
    Chapter 15 Protein Engineering
    15.1 Introduction
    15.2 Tools of the Trade
    15.3 Applications
    15.4 Conclusions and Future Directions References
    Chapter 16 Immobilisation of Enzymes and Cells
    16.1 Introduction
    16.2 Biocatalysts
    16.3 Immobilisation
    16.4 Properties of Immobilised Biocatalysts
    16.5 Applications
    References
    Chapter 17 Downstream Processing
    17.1 Introduction
    17.2 Initial Considerations and Primary Recovery
    17.3 Protein Precipitation
    17.4 Chromatography
    17.5 Alternatives to Packed Bed Chromatography
    17.6 Design of Biomolecules for Downstream Processing
    17.7 Scaledown Methods
    17.8 Validation and Robustness
    17.9 Formulation and Antiviral Treatments
    17.10 Current Developments and Future Directions
    References
    Chapter 18 Biosensors
    18.1 Introduction
    18.2 The Biological Reaction
    18.3 Theory
    18.4 Electrochemical Methods
    18.5 Piezoelectric Biosensors
    18.6 Optical Biosensors
    18.7 Whole Cell Biosensors
    18.8 Receptor-based Sensors
    18.9 Conclusion
    References
    Chapter 19 Biofuels and Biotechnoiogy
    19.1 Introduction
    19.2 Production of the Major Biofuels
    19.3 Application of Biotechnology Tools to Biofuels Processes
    19.4 Future Perspectives
    References
    Subject Index