Zebrafish : methods and protocols / edited by Graham J. Lieschke, Andrew C. Oates and Koichi Kawakami. — [Totowa, N.J.] : Humana ; London : Springer [distributor], c2009. – (58.17/M592/v.546) |
Contents
Contents
PART I MUTAGENESIS AND MUTANTS
1. Highly Efficient ENU Mutagenesis in Zebrafish
2. Production of Pseudotyped Retrovirus and the Generation of Proviral Transgenic Zebrafish
3. Undertaking a Successful Gynogenetic Haploid Screen in Zebrafish
4. Cryopreservation and In Vitro Fertilization at the Zebrafish International Resource Center
PART II TRANSGENESIS
5. Transient and Stable Transgenesis Using Tol2 Transposon Vectors
6. Analysis of Genes and Genome by the Tol2-Mediated Gene and Enhancer Trap Methods
7. Bacterial Artificial Chromosome Transgenesis for Zebrafish 103
8. Simple and Efficient Transgenesis with Meganuclease Constructs in Zebrafish 117
PART III TISSUE-SPECIFIC MANIPULATIONS
9. Nitroreductase-Mediated Cell Ablation in Transgenic Zebrafish Embryos
10. Focal Electroporation in Zebrafish Embryos and Larvae
11. Tissue Micromanipulation in Zebrafish Embryos
PART IV ANALYZING GENE EXPRESSION
12. Zebrafish Spotted-Microarray for Genome-Wide Expression Profiling Experiments.
Part I: Array Printing and Hybridization
13. Zebrafish Spotted-Microarray for Genome-Wide Expression Profiling Experiments: Data Acquisition and Analysis 197
14. Validating microRNA Target Transcripts Using Zebrafish Assays 227
PART V IMAGING
15. Multiple Embryo Time-Lapse Imaging of Zebrafish Development 243
16. Live Cell Imaging of Zebrafish Leukocytes 255
17. Imaging Zebrafish Embryos by Two-Photon Excitation Time-Lapse Microscopy 273
18. Morphological Analysis of the Zebrafish Digestive System 289
19. In Toto Imaging of Embryogenesis with Confocal Time-Lapse Microscopy 317
Index