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Proteomics : methods and protocols / edited by Jorg Reinders and Albert Sickmann. — Dordrecht ;London : Humana Press, 2009. – (58.17/M592/v.564) |
Contents
Contents
PART I INTRODUCTION
1. Introduction to Proteomics
PART II ELECTROPHORETIC SEPARATIONS
2. High-Resolution Two-Dimensional Electrophoresis
3. Non-classical 2-D Electrophoresis
4. Protein Detection and Quantitation Technologies for Gel-Based Proteome Analysis
PART III MASS SPECTROMETRY AND TANDEM MASS SPECTROMETRY APPLICATIONS
5. MALDI MS
6. Capillary Electrophoresis Coupled to Mass Spectrometry for Proteomic Profiling of Human Urine and Biomarker Discovery
7. A Newcomer's Guide to Nano-Liquid-Chromatography of Peptides 123
8. Multidimensional Protein Identification Technology
9. Characterization of Platelet Proteins Using Peptide Centric Proteomics 15 5
10. Identification of the Molecular Composition of the 20S Proteasome of Mouse Intestine by High-Resolution Mass Spectrometric Proteome Analysis 173
PART IV QUANTITATIVE PROTEOMICS
11. Liquid Chromatography-Mass Spectrometry-Based Quantitative Proteomics 189
12. iTRAQ-Labeling of In-Gel Digested Proteins for Relative Quantification 207
13. Electrospray Mass Spectrometry for Quantitative Plasma Proteome Analysis 227
PART V INTERPRETATION OF MASS SPECTROMETRY DATA
14. Algorithms and Databases 245
15. Shotgun Protein Identification and Quantification by Mass Spectrometry 261
PART VI ANALYSIS OF PROTEIN MODIFICATIONS
16. Proteomics Identification of Oxidatively Modified Proteins in Brain
17. Isotope-Labeling and Affinity Enrichment of Phosphopeptides for Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry
PART VII SUBCELLULAR PROTEOMICS
18. Organelle Proteomics: Reduction of Sample Complexity by Enzymatic In-Gel Selection of Native Proteins 325
19. Isolation of Plasma Membranes from the Nervous System by Countercurrent Distribution in Aqueous Polymer Two-Phase Systems 335
20. Enrichment and Preparation of Plasma Membrane Proteins from Arabidopsis thaliana for Global Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry 341
PART VIII ANALYSIS OF PROTEIN INTERACTIONS
21. Tandem Affinity Purification of Protein Complexes from Mammalian Cells by the Strep/FLAG (SF)-TAP Tag 359
22. Sequential Peptide Affinity Purification System for the Systematic Isolation and Identification of Protein Complexes from Escherichia coli 373
23. Bioinformatical Approaches to Detect and Analyze Protein Interactions 401
Index 433
