 |
Protein blotting and detection : methods and protocols / edited by Biji T. Kurien and R. Hal Scofield. — [Totowa, N.J.] :London : Humana ;Springer [distributor], c2009. – (58.17/M592/v.536) |
Contents
Contents
Preface
Contributors
1. Origins of Protein Blotting
2. Western Blotting: Remembrance of Past Things
3. Introduction to Protein Blotting
4. Simultaneous Immunoblotting Analysis with Activity Gel Electrophoresis and 2-D Gel Electrophoresis
5. Diffusion Blotting for Rapid Production of Multiple Identical Imprints from Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis on a Solid Support
6. Tryptic Peptide Purification Using Polyvinylidene Difluoride Membrane for Matrix-Assisted Laser Dcsorption Ionization Time of Flight Mass Spectrometry
7. Affinity Immunoblotting for Analysis of Antibody Clonotype Distribution in a Lupus Patient Developing Anti-Ro 60 Over Time
8. Non-electrophoretic Bi-dircctional Transfer of a Single SDS-PAGE Gel with Multiple Antigens to Obtain 12 hnmunoblots
9. Slice Blotting
10. Localizing Proteins by Tissue Printing
11. Dot-Immunobinding Assay
12. Capillary Blotting of Glycosaminoglycans on Nitrocellulose Membranes After Agarose Gel Electrophoresis Separation
13. Quantitative Computerized Western Blotting
14. Cationic Electrophoresis and Eastern Blotting 115
15. A Miniaturized Blotting System for Simultaneous Detection of Different Autoantibodies 129
16. Transfer and Multiplex Immunoblotting of a Paraffin Embedded Tissue 139
17. Multistrip Western Blotting to Increase Quantitative Data Output 149
18. Western Blotting/Edman Sequencing using PVDF Membrane 163
19. Blotting from PhastGel to Membranes by Ultrasound 173
20. Ultrarapid Electrophoretic Transfer of High and Low Molecular Weight Proteins Using Heat 181
21. Strip Immunoblotting of Multiple Antigenic Peptides to Nitrocellulose Membrane 191
22. Affinity Purification of Autoantibodics from an Antigen Strip Excised from a Nitrocellulose Protein Blot 201
23. Double-Blotting: A Solution to the Binding of Secondary Antibodies in Problem of Nonspecific Immunoblotting Procedures 213
24. Efficient Electroblotting of Very Large Proteins Using a Vertical Agarose Electrophoresis System
25. Semi-Dry Protein Transfer and Immunodctection of P-Selectin Using an Antibody to its C-terminal Tag
26. The Multiple Antigen Blot Assay: A Simple， Versatile and Multipurpose Immunoenzymatic Technique 237
27. Blotting from Immobilized pH Gradient Gels: Application to Total Cell Lysates 253
28. Immunoprecipitation-Western Blot for Proteins of Low Abundance 259
29. Native Electrophoresis and Western Blot Analysis: Method and Applications 277
30. Grid-Immunoblotting 289
31. Sequential Use of Immunoblots for Characterization of Autoantibody Specificities 293
32. Renaturation of Recombinant Ro 60 Autoantigen by Calcium Ions on PVDF Membrane 299
33. Intermittent Microwave Irradiation Facilitates Antigen-Antibody Reaction in Western Blot Analysis
34. Detection of Protein-Protein Interactions by Far-Western Blotting
35. On-Membrane Tryptic Digestion of Proteins for Mass Spectrometry Analysis 331
36. Western Blot Analysis of Adhesive Interactions under Fluid Shear Conditions: The Blot Roiling Assay 343
37. ELISPOT Assay on Membrane Microplates
38. A Brief Review of Other Notable Protein Blotting Methods
39. Phosphoprotein Detection on Protein Electroblot Using a Phosphate-Specific Fluorophore 385
40. Rapid， Antibody-Free Detection of Recombinant Proteins on Blots Using Enzyme Fragment Complementation 395
41. Fluorescent Labeling of Proteins and Its Application to SDS-PAGE and Western Blotting 407
42. Whole and Strip Nitrocellulose Membrane as well as New Line Immunoblotting of Antigen Using the Chemiluminescence Technique 417
43. Protein Stains to Detect Antigen on Membranes 433
44. Use of Nonradioactive Detection Method for North- and Southwestern Blot
45. Immunoblotting Using Radiolabeled Reagents for Detection 451
46. Detection of Protein Carbonyls by Means of Biotin Hydrazide-Streptavidin Affinity Methods 457
47. "Rainbow" Western Blotting 463
48. Multiple Antigen Detection Western Blotting 473
49. Detection of Calcium Binding by Ro 60 Multiple Antigenic Peptides on PVDF Membrane Using Quin-2 483
50. Detection of La/SS-B by Western Blot Using Nanogold-Tagged Antibodies and Silver Enhancement 491
51. Imaging Systems for Westerns: Chemiluminescence vs. Infrared Detection 499
52. A Fluorescent Codetection System for Immunoblotting and Proteomics Through ECL-Plex and CyDye Labeling 515
53. Visualization of Unstained Protein Bands on PVDF 527
54. Improved Protein Detection Using Cold Microwave Technology.
55. TLC Blot (Far-Eastern Blot) and Its Applications 545
56. A Brief Review of Other Notable Protein Detection Methods on Blots 557
57. Sending Secret Messages on Nitrocellulose Membrane and the Use of a Molecular Pen for Orientation in ECL Membrane Assays
Index 583
