 |
Glycomics : methods and protocols / edited by Nicolle H. Packer and Niclas G. Karlsson. — Totowa, N.J. :[London : Humana ;Springer, distributor], 2009. – (58.17/M592/v.534) |
Contents
Contents
SECTION I: GLYCAN ANALYSIS
SUBSECTION A: RELEASED GLYCANS
1 Analysis of N- and O-Linked Glycans from Glycoproteins Using MALDI-TOF Mass Spectrometry 5
2 Infrared Multiphoton Dissociatkm Mass Spectrometry for Structural Elucidation of Oligosaccharides 23
3 Mass Spectrometry of N-Linked Glycans
4 Solid-Phase Permethylation for Glycomic Analysis 53
5 Method for Investigation of Oligosaccharides Using Phenylhydrazine Derivatization 65
6 Two-Dimensional HPLC Separation with Reverse-Phase-Nano-LC-MS/MS for the Characterization of Glycan Pools After Labeling with 2-Aminobenzamide 79
7 Capillary Lectin-Affinity Electrophoresis for Glycan Analysis 93
8 Analysis of Methylated O-Glycan Alditols by Reversed-Phase NanoLC Coupled CAD-ESI Mass Spectrometry 107
9 High-Throughput and High-Sensitivity Nano-LC/MS and MS/MS for O-Glycan Profiling 117
10 Collision-Induced Dissociation Tandem Mass Spectrometry for Structural Elucidation of Glycans 133
11 The Structural Elucidation of Glycosaminoglycans 147
12 Labelling Heparan Sulphate Saccharides with Chromophore， Fluorescence and Mass Tags for HPLC and MS Separations 157
13 Small-Scale Enzymatic Digestion of Glycoproteins and Proteoglycans for Analysis of Oligosaccharides by LC-MS and FACE Gel Electrophoresis 171
SUBSECTION B: GLYCOPEPTIDES
14 Enrichment Strategies for Glycopeptides
15 Introductory Glycosylation Analysis Using SDS-PAGE and Peptide Mass Fingerprinting
16 Characterization of N-Linked Glycosylation on Recombinant Glycoproteins Produced in Pichia pastoris Using ESI-MS and MALDI-TOF 213
17 N-Glycosylation Site Analysis of Human Platelet Proteins by Hydrazide Affinity Capturing and LC-MS/MS 225
18 LC/MS" for Glycoprotein Analysis: N-Linked Glycosylation Analysis and Peptide Sequencing of Glycopeptides 239
SUBSECTION C: O-GlcNAcomics
19 Detecting the "O-GlcNAcome"; Detection， Purification， and Analysis of O-GlcNAc Modified Proteins
SUBSECTION D: GLYCOBIOINFORMATIC TOOLS
20 Preparation of a Glycan Library Using a Variety of Glycosyltrasferases 283
21 Data Mining the PDB for Glyco-Related Data
SECTION II: CARBOHYDRATE-PROTEIN INTERACTIONS
22 Saccharide Microarrays for High-Throughput Interrogation of Glycan-Protein Binding Interactions 313
23 Glycosaminoglycan Characterization Methodologies: Probing Biomolecular Interactions 331
24 Development and Characterization of Antibodies to Carbohydrate Antigens 341
25 Inhibition of Glycosyltransferase Activities as the Basis for Drug Development 359
26 Saturation Transfer Difference NMR Spectroscopy as a Technique to Investigate Protein-Carbohydrate Interactions in Solution
