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Toll-like receptors : methods and protocols / edited by Claire E. McCoy and Luke A.J. ONeill. — Totowa, N.J. : Humana ; [London : Springer, distributor], c2009. – (58.17/M592/v.517)

Contents

    Contents
    
    Contributors
    PART I: METHODS TO DETECT AND ANALYZE TOLL-LIKE RECEPTORS
    1 Expression Analysis of the Toll-Like Receptors in Human Peripheral Blood Mononuclear Cells
    2 Ligands, Cell-Based Models, and Readouts Required for Toll-Like Receptor Action
    3 Toll-Like Receptor Interactions Imaged by FRET Microscopy and GFP Fragment Reconstitution
    4 Predicting Toll-Like Receptor Structures and Characterizing Ligand Binding
    5 Bioinformatic Analysis of Toll-Like Receptor Sequences and Structures
    6 Expression, Purification, and Crystallization of Toll/Interleukin- 1 Receptor (TIR) Domains
    PART II: METHODS TO ANALYZE SIGNAL TRANSDUCTION DOWNSTREAM OF TOLL-LIKE RECEPTOR STIMULATION
    7 Proteomic Analysis of Protein Complexes in Toll-Like Receptor Biology
    8 2D-DIGE: Comparative Proteomics of Cellular Signalling Pathways 105
    9 MAPPIT (Mammalian Protein-Protein Interaction Trap) Analysis of Early Steps in Toll-Like Receptor Signalling
    10 Analysis of the Functional Role of Toll-Like Receptor-4 Tyrosine Phosphorylation
    11 Analysis of Ubiquitin Degradation and Phosphorylation of Proteins
    12 The Generation of Highly Purified Primary Human Neutrophils and Assessment of Apoptosis in Response to Toll-Like Receptor Ligands 191
    13 Cellular Expression of A20 and ABIN-3 in Response to Toll-Like Receptor-4 Stimulation 205
    14 Characterisation of Viral Proteins that Inhibit Toll-Like Receptor Signal Transduction 217
    PART III: GENETIC TECHNIQUES IN TOLL-LIKE RECEPTOR ANALYSIS
    15 Genetic Dissection of Toll-Like Receptor Signaling Using ENU Mutagenesis 239
    16 Microarray Experiments to Uncover Toll-Like Receptor Function 253
    17 Uncovering Novel Gene Function in Toll-Like Receptor Signalling Using siRNA 277
    18 Genotyping Methods to Analyse Polymorphismsin Toll-Like Receptors and Disease 297
    PART IV: TOOL-LIKE RECEPTORS AND DISEASE
    19 Experimental Models of Acute Infection and Toll-Like Receptor Driven Septic Shock 313
    20 Toll-Like Receptors and Rheumatoid Arthritis
    21 Practical Techniques for Detection of Toll-Like Receptor-4 in the Human Intestine
    22 Toll-Like Receptor-Dependent Immune Complex Activation of B Cells and Dendritic Cells
    23 Innate Immunity, Toll-Like Receptors, and Atherosclerosis: Mouse Models and Methods 381
    24 Generation of Parasite Antigens for Use in Toll-Like Receptor Research 401
    25 Biomarkers Measuring the Activity of Toll-Like Receptor Ligands in Clinical Development Programs
    Index