Host-pathogen interactions : methods and protocols / edited by Steffen Rupp and Kai Sohn. — Totowa, N.J. : Humana ; London : Springer, distributor, c2009. – (58.17/M592/v.470)

Contents

    Contents
    
    Preface v
    Contributors ix
    PART I: BACTERIAL PATHOGENS
    1 Introduction: Bacterial Pathogens
    2 Transcriptome Analyses in the Interaction of Neisseria meningitidis with Mammalian Host Cells
    3 Surface-Exposed Adherence Molecules of Streptococcuspneumoniae
    4 Screening of Virulence Traits in Legionella pneumophila and Analysis of the Host Susceptibility to Infection by Using the Dictyostelium Host Model System
    5 Characterizing Host Receptor Recognition by Individual Bacterial Pathogens
    PART II: FUNGAL PATHOGENS
    6 Introduction: Fungal Pathogens
    7 Isolation and Purification of Antigenic Components of Cryptococcus
    8 A Method for Examining Glycans Surface Expression of Yeasts by Flow Cytometry
    9 Human Epithelial Model Systems for the Study of Candida Infections In Vitro: Part I. Adhesion to Epithelial Models
    10 Human Epithelial Model Systems for the Study of Candida Infections In Vitro: Part II. Histologic Methods for Studying Fungal Invasion
    11 In Vitro Systems for Studying the Interaction of Fungal Pathogens with Primary Cells from the Mammalian Innate Immune System
    12 Experimental Infection of Rodent Mammals for Fungal Virulence Testing
    13 Real-Time and Semiquantitative RT-PCR Methods to Analyze Gene Expression Patterns During Aspergillus-Host Interactions
    14 Antibody-Based Strategy to Identify Candida albicans Genes Expressed During Infections
    15 Identification of the Candida albicans Immunome During Systemic Infection by Mass Spectrometry
    PART III: PARASITES
    16 Introduction: Parasites
    17 Axenic In Vitro Cultivation of Echinococcus multilocularis Metacestode Vesicles and the Generation of Primary Cell Cultures
    18 Culture of Exoerythrocytic Stages of the Malaria Parasites Plasmodium falciparum and Plasmodium vivax
    19 Modulation of Caspase Activation by Toxoplasmagondii
    PART IV: HOST RESPONSES
    20 Introduction: Host Responses
    21 Fungal and Bacterial Killing by Neutrophils
    22 Endothelial Cell Stimulation by Candida albicans
    23 Models of Oral and Vaginal Candidiasis Based on In Vitro Reconstituted Human Epithelia for the Study of Host-Pathogen Interactions
    24 Phagocytosis of Candida albicans by RNAi-Treated Drosophila $2 Cells
    25 Oral Mucosal Cell Response to Candida albicans in Transgenic Mice Expressing HIV-1
    26 Proteomic Profiling of Serologic Response to Candida albicans During Host-Commensal and Host-Pathogen Interactions
    Index 413