Type 2 diabetes : methods and protocols / edited by Claire Stocker. — Totowa, N.J. ; London : Humana, 2009

Contents

    Contents
    
    1. Selecting the "Right" Mouse Model for Metabolic Syndrome and Type 2 Diabetes Research
    2. Nutritional Models of Type 2 Diabetes Mellitus
    3. The Isolation and Purification of Rodent Pancreatic Islets of Langerhans
    4. The Measurement of Insulin Secretion from Isolated Rodent Islets of Langerhans
    5. The Incubation and Monitoring of Cell Viability in Primary Rat Islets of Langerhans and Pancreatic 13-Cell Lines
    6. In Vitro Culture of Isolated Islets of Langerhans: Analysis of Function
    7. Single-Cell RT-PCR Identification of Genes Expressed by Human Islet Endocrine Cells
    8. Laser Capture Microdissection of Human Pancreatic β-Cells and RNA Preparation for Gene Expression Profiling
    9. In Vitro Transdifferentiation of Human Hepatoma Cells into Pancreatic-Like Cells
    10. The Measurement of GLUT4 Translocation in 3T3-L1 Adipocytes 111
    11. Pancreatic Remodeling: Beta-Cell Apoptosis， Prolifkration and Neogenesis， and the Measurement of Beta-Cell Mass and of Individual Beta-Cell Size 137
    12. Morphology of Pancreatic Islets: A Time Course of Pre-diabetes in Zucker Fatty Rats 159
    13. Fluorescent Immunohistochemistry and In Situ Hybridization Analysis of Pancreas 191
    14. The Measurement of Insulin Secretion Using Pancreas Perfusion in the Rodent
    15. Hyperinsulinemic-Euglycemic Clamp to Assess Insulin Sensitivity In Vivo 221
    16. Gene Expression Analysis in Diabetes Research 239
    17. Gene Expression Mining in Type 2 Diabetes Research 263
    Index