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Membrane proteomics : methods and protocols / edited by Matthew J. Peirce and Robin Wait. — [Totowa, N.J.] : Humana, c2009. – (58.17/M592/v.528)

Contents

    Contents
    
    Preface v
    Contributors xi
    PART I. In Silico METHODS FOR PREDICTION OF MEMBRANE PROTEIN HYDROPHOBICITY AND TOPOLOGY
    1 Online Tools for Predicting Integral Membrane Proteins
    2 In Silico Identification of Novel G Protein Coupled Receptors
    3 Transcriptome-Based Identification of Candidate Membrane Proteins
    PART II. EXTRACTION AND PURIFICATION OF MEMBRANE PROTEINS
    PART A. PLANT MEMBRANE PROTEINS
    4 Separation of Thylakoid Membrane Proteins by Sucrose Gradient Ultracentrifugation or Blue Native-SDS-PAGE Two-Dimensional Electrophoresis
    PART B. PROKARYOTIC MEMBRANE PROTEINS
    5 Extraction of Yeast Mitochondrial Membrane Proteins by Solubilization and Detergent/Polymer Aqueous Two-Phase Partitioning
    6 16-BAC/SDS-PAGE Analysis of Membrane Proteins of Yeast Mitochondria Purified by Free Flow Electrophoresis
    PART C. MAMMALIAN MEMBRANE PROTEINS
    7 Sequential Detergent Extraction Prior to Mass Spectrometry Analysis
    8 Enrichment of Brain Plasma Membranes by Affinity Two-Phase Partitioning
    9 Protocol to Enrich and Analyze Plasma Membrane Proteins
    10 Proteomic Analysis of the Lymphocyte Plasma Membrane Using Cell Surface Biotinylation and Solution-Phase Isoelectric Focusing
    11 Identification of Target Membrane Proteins as Detected by Phage Antibodies
    12 Membrane Protease Degradomics: Proteomic Identification and Quantification of Cell Surface Protease Substrates
    13 Purification of Basolateral Integral Membrane Proteins by Cationic Colloidal Silica-Based Apical Membrane Subtraction
    14 Moving Closer to the Lipid Raft Proteome Using Quantitative Proteomics
    15 Use of Sequential Chemical Extractions to Purify Nuclear Membrane Proteins for Proteomics Identification
    16 Isolation of Extracellular Membranous Vesicles
    PART III. SEPARATION OF MEMBRANE PROTEINS
    17 Enrichment of Human Platelet Membranes for Proteomic Analysis
    18 Detergents and Chaotropes for Protein Solubilization Before Two-Dimensional Electrophoresis
    19 Two-Dimensional Separation of Membrane Proteins by 16-BAC-SDS-PAGE
    PART IV. IDENTIFICATION AND QUANTIFICATION OF MEMBRANE PROTEINS
    20 MudPIT Analysis: Application to Human Heart Tissue
    21 Liquid Chromatography MALDI MS/MS for Membrane Proteome Analysis
    22 Cysteinyl-Tagging of Integral Membrane Proteins for Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry
    23 Quantitative Proteomics Analysis of Pancreatic Zymogen Granule Membrane Proteins
    Index 339