Membrane proteomics : methods and protocols / edited by Matthew J. Peirce and Robin Wait. — [Totowa, N.J.] : Humana, c2009. – (58.17/M592/v.528) |
Contents
Contents
Preface v
Contributors xi
PART I. In Silico METHODS FOR PREDICTION OF MEMBRANE PROTEIN HYDROPHOBICITY AND TOPOLOGY
1 Online Tools for Predicting Integral Membrane Proteins
2 In Silico Identification of Novel G Protein Coupled Receptors
3 Transcriptome-Based Identification of Candidate Membrane Proteins
PART II. EXTRACTION AND PURIFICATION OF MEMBRANE PROTEINS
PART A. PLANT MEMBRANE PROTEINS
4 Separation of Thylakoid Membrane Proteins by Sucrose Gradient Ultracentrifugation or Blue Native-SDS-PAGE Two-Dimensional Electrophoresis
PART B. PROKARYOTIC MEMBRANE PROTEINS
5 Extraction of Yeast Mitochondrial Membrane Proteins by Solubilization and Detergent/Polymer Aqueous Two-Phase Partitioning
6 16-BAC/SDS-PAGE Analysis of Membrane Proteins of Yeast Mitochondria Purified by Free Flow Electrophoresis
PART C. MAMMALIAN MEMBRANE PROTEINS
7 Sequential Detergent Extraction Prior to Mass Spectrometry Analysis
8 Enrichment of Brain Plasma Membranes by Affinity Two-Phase Partitioning
9 Protocol to Enrich and Analyze Plasma Membrane Proteins
10 Proteomic Analysis of the Lymphocyte Plasma Membrane Using Cell Surface Biotinylation and Solution-Phase Isoelectric Focusing
11 Identification of Target Membrane Proteins as Detected by Phage Antibodies
12 Membrane Protease Degradomics: Proteomic Identification and Quantification of Cell Surface Protease Substrates
13 Purification of Basolateral Integral Membrane Proteins by Cationic Colloidal Silica-Based Apical Membrane Subtraction
14 Moving Closer to the Lipid Raft Proteome Using Quantitative Proteomics
15 Use of Sequential Chemical Extractions to Purify Nuclear Membrane Proteins for Proteomics Identification
16 Isolation of Extracellular Membranous Vesicles
PART III. SEPARATION OF MEMBRANE PROTEINS
17 Enrichment of Human Platelet Membranes for Proteomic Analysis
18 Detergents and Chaotropes for Protein Solubilization Before Two-Dimensional Electrophoresis
19 Two-Dimensional Separation of Membrane Proteins by 16-BAC-SDS-PAGE
PART IV. IDENTIFICATION AND QUANTIFICATION OF MEMBRANE PROTEINS
20 MudPIT Analysis: Application to Human Heart Tissue
21 Liquid Chromatography MALDI MS/MS for Membrane Proteome Analysis
22 Cysteinyl-Tagging of Integral Membrane Proteins for Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry
23 Quantitative Proteomics Analysis of Pancreatic Zymogen Granule Membrane Proteins
Index 339