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新书资源(2009年7月)

Two dimensional electrophoresis protocols / edited by David Sheehan and Raymond Tyther. — Totowa, N.J. : Humana, c2009. – (58.17/M592/v.519)

Contents

    Contents
    
    Preface
    Contributors
    OVERVIEW CHAPTERS
    1 Two-Dimensional Electrophoresis: An Overview
    2 Solubilization of Proteins in 2DE: An Outline
    3 Selection of pH Ranges in 2DE 31
    4 Difficult Proteins
    5 Organelle Proteomics 65
    6 Applications of Chemical Tagging Approaches in Combination with 2DE and Mass Spectrometry 83
    7 Immunoblotting 2DE Membranes
    PROTOCOLS CHAPTERS
    8 Troubleshooting Image Analysis in 2DE 113
    9 Analysis of Bacterial Proteins by 2DE 131
    10 Proteomic Analysis of Caenorhabditis elegans
    11 Protein Extraction for 2DE
    12 Analysis of Proteins from Marine Molluscs
    13 Preparation and Analysis of Plastid Proteomes by 2DE
    14 High-Resolution 2DE
    15 Blue Native-Gel Electrophoresis Proteomics
    16 2DE for Proteome Analysis of Human Metaphase Chromosomes
    17 Microsomal Proteomics
    18 Prefractionation Using Microscale Solution IEF
    19 Diagonal Electrophoresis for Detection of Protein Disulphide Bridges
    20 High-Resolution Large-Gel 2DE
    21 Silver Staining of Proteins in 2DE Gels
    22 Detection of 4-Hydroxy-2-Noncnal- and 3-Nitrotyrosine-Modified Proteins Using a Proteomics Approach
    23 Proteomic Detection of Oxidized and Reduced Thiol Proteins in Cultured Cells
    24 Detection of Ubiquitination in 2DE
    25 Phosphoproteome Analysis by In-Gel Isoelectric Focusing and Tandem Mass Spectrometry
    26 Detection of Protein Glutathionylation
    27 Activity-Based Protein Profiling of Protein Tyrosine Phosphatases 417
    28 Active Protease Mapping in 2DE Gels
    29 Two-Dimensional Difference Gel Electrophoresis
    30 Protein Expression Profiling
    31 C-Terminal Sequence Analysis of 2DE-Separated Proteins
    32 Shotgun Protein Analysis by Liquid Chromatography-Tandem Mass Spectrometry
    33 De Novo Sequence Analysis of N-Terminal Sulfonated Peptides After in-Gel Guanidination 495
    34 Tryptic Digestion of In-Gel Proteins for Mass Spectrometry Analysis 507
    35 Database Interrogation Algorithms for Identification of Proteins in Proteomic Separations 515
    36 Creating 2DE Databases for the World Wide Web 533
    Index