Two dimensional electrophoresis protocols / edited by David Sheehan and Raymond Tyther. — Totowa, N.J. : Humana, c2009. – (58.17/M592/v.519) |
Contents
Contents
Preface
Contributors
OVERVIEW CHAPTERS
1 Two-Dimensional Electrophoresis: An Overview
2 Solubilization of Proteins in 2DE: An Outline
3 Selection of pH Ranges in 2DE 31
4 Difficult Proteins
5 Organelle Proteomics 65
6 Applications of Chemical Tagging Approaches in Combination with 2DE and Mass Spectrometry 83
7 Immunoblotting 2DE Membranes
PROTOCOLS CHAPTERS
8 Troubleshooting Image Analysis in 2DE 113
9 Analysis of Bacterial Proteins by 2DE 131
10 Proteomic Analysis of Caenorhabditis elegans
11 Protein Extraction for 2DE
12 Analysis of Proteins from Marine Molluscs
13 Preparation and Analysis of Plastid Proteomes by 2DE
14 High-Resolution 2DE
15 Blue Native-Gel Electrophoresis Proteomics
16 2DE for Proteome Analysis of Human Metaphase Chromosomes
17 Microsomal Proteomics
18 Prefractionation Using Microscale Solution IEF
19 Diagonal Electrophoresis for Detection of Protein Disulphide Bridges
20 High-Resolution Large-Gel 2DE
21 Silver Staining of Proteins in 2DE Gels
22 Detection of 4-Hydroxy-2-Noncnal- and 3-Nitrotyrosine-Modified Proteins Using a Proteomics Approach
23 Proteomic Detection of Oxidized and Reduced Thiol Proteins in Cultured Cells
24 Detection of Ubiquitination in 2DE
25 Phosphoproteome Analysis by In-Gel Isoelectric Focusing and Tandem Mass Spectrometry
26 Detection of Protein Glutathionylation
27 Activity-Based Protein Profiling of Protein Tyrosine Phosphatases 417
28 Active Protease Mapping in 2DE Gels
29 Two-Dimensional Difference Gel Electrophoresis
30 Protein Expression Profiling
31 C-Terminal Sequence Analysis of 2DE-Separated Proteins
32 Shotgun Protein Analysis by Liquid Chromatography-Tandem Mass Spectrometry
33 De Novo Sequence Analysis of N-Terminal Sulfonated Peptides After in-Gel Guanidination 495
34 Tryptic Digestion of In-Gel Proteins for Mass Spectrometry Analysis 507
35 Database Interrogation Algorithms for Identification of Proteins in Proteomic Separations 515
36 Creating 2DE Databases for the World Wide Web 533
Index