Microinjection : methods and applications / edited by David J. Carroll. — New York, N.Y. : Humana, c2009. – (58.17/M592/v.518)

Contents

    Contents
    
    1 Expression of Exogenous mRNA in Xenopus laevis Embryos for the Study of Cell Cycle Regulation
    2 Use of Luciferase Chimaera to Monitor PLCζ Expression in Mouse Eggs
    3 Analysis of 14-3-3 Family Member Function in Xenopus Embryos by Microinjection ofAntisense Morpholino Oligos
    4 A Microinjectable Biological System， the Xenopus Oocyte， as an Approach to Understanding Signal Transduction Protein Function
    5 Combining Microinjection and Immunoblotting to Analyze MAP Kinase Phosphorylation in Single Starfish Oocytes and Eggs
    6 Analysis of Signaling Pathways in Zebrafish Development by Microinjection 67
    7 Protein Inhibition by Microinjection and RNA-Mediated Interference in Tissue Culture Cells: Complementary Approaches to Study Protein Function
    8 DNA Delivery by Microinjection for the Generation of Recombinant Mammalian Cell Lines 99
    9 Bacteriophage qbC31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels
    10 Germline Transformation of Caenorhabditis elegans by Injection
    11 Quantitative Microinjection of Mouse Oocytes and Eggs
    12 Microinjection of Follicle-Enclosed Mouse Oocytes
    13 Functional Studies of Regulatory Genes in the Sea Urchin Embryo
    14 Exploring the Cytoskeleton During Intracytoplasmic Sperm Injection in Humans
    15 Somatic Cell Nuclear Transfer in the Mouse
    Index