 |
Angiogenesis protocols / edited by Stewart Martin and Cliff Murray. — 2nd ed. — New York, N.Y. : Humana, c2009. – (58.17/M592/v.467) |
Contents
Contents
Preface
Contributors
SECTION I: REVIEW ARTICLES
1. Angiogenic Signalling Pathways
2. Imaging Angiogenesis 25
SECTION II: MICROSCOPIC ASSESSMENT
3. Assessing Tumor Angiogenesis in Histological Samples 55
4. Immunohistochernical Methods for Measuring Tissue Lymphangiogenesis
SECTION III: CELL ISOLATION TECHNIQUES
5. Vascular Endothelial Cells from Human Micro- and Macrovessels: Isolation， Characterisation and Culture
6. Lymphatic Endothelial Cells: Establishment of Primaries and Characterization of Established Lines 113
7. Isolation， Culture and Characterisation of Vascular Smooth Muscle Cells
8. Circulating Endothelial Progenitor Cells 139
SECTION IV: IN VITRO TECHNIQUES
9. In Vitro Assays for Endothelial Cell Functions Related to Angiogenesis: Proliferation， Motility， Tubular Differentiation， and Proteolysis
10. Tube Formation: An In Vitro Matrigel Angiogenesis Assay 183
11. Three-Dimensional In Vitro Angiogenesis in the Rat Aortic Ring Model 189
12. Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature
13. Genetic Manipulation of Corneal Endothelial Cells: Transfection and Viral Transduction 229
14. Creation of Human Skin Equivalents for the In Vitro Study of Angiogenesis in Wound Healing 241
SECTION V: 1N VIVO TECHNIQUES
15. Measurement of Angiogenic Phenotype by Use of Two- Dimensional Mesenteric Angiogenesis Assay 251
16. Quantitative Estimation of Tissue Blood Flow Rate 271
17. In Vivo Matrigel Migration and Angiogenesis Assay 287
18. The Sponge Implant Model of Angiogenesis 295
19. The Dorsal Skinfold Chamber: Studying Angiogenesis by Intravital Microscopy 305
20. The Corneal Pocket Assay 319
21. Use of the Hollow Fibre Assay for Studies of Tumor Neovasculature 331
22. The Cranial Bone Window Model: Studying Angiogenesis of Primary and Secondary Bone Tumors by Intravital Microscopy 343
Index 357
