Gene knockout protocols / edited by Ralf Kuhn, Wolfgang Wurst. — 2nd ed. — [Totowa, N.J.] : Humana, c2009. – (58.17/M592/v.530) |
Contents
Contents
Preface
Contributors
Color Plates
1. Overview on Mouse Mutagenesis
PART I: GENE MODIFICATION IN ES CELLS
2. Construction of Gene-Targeting Vectors by Recombineering
3. Gene-Trap Vectors and Mutagenesis
4. Chromosome Engineering in ES Cells 49
5. Gene Modification in Embryonic Stem Cells by Single-Stranded DNA Oligonucleotides
6. Generation of shRNA Transgenic Mice
7. Mutagenesis of Mouse Embryonic Stern Cells with Ethylmethanesulfonate 131
PART II: STEM CELL MANIPULATION
8. Gene Targeting in Mouse Embryonic Stem Cells 141
9. Manipulating Mouse Embryonic Stem Cells 165
10. ES Cell Line Establishment
11. Generation of Double-Knockout Embryonic Stem Cells 205
12. Differentiation Analysis of Pluripotent Mouse Embryonic Stem (ES) Cells In Vitro 219
13. Cloning of ES Cells and Mice by Nuclear Transfer 251
PART III: GENETICALLY ENGINEERED Mice
14. Isolation, Microinjection and Transfer of Mouse Blastocysts
15. Aggregation Chimeras: Combining ES Cells, Diploid, and Tetraploid Embryos
16. VelociMouse: Fully ES Cell-Derived F0-Generation Mice Obtained from the Injection of ES Cells into Eight-Cell-Stage Embryos 311
17. Generation of Cre Recombinase-Expressing Transgenic Mice Using Bacterial Artificial Chromosomes 325
18. Inducible Cre Mice
19. Creation and Use of a Cre Recombinase Transgenic Database
20. Transposon Mutagenesis in Mice
21. Lentiviral Transgenesis 391
22. Sperm Cryopreservation and In Vitro Fertilization
PART IV: PHENOTYPE ANALYSIS23. Influence of Genetic Background on Genetically Engineered Mouse Phenotypes
24. Pathologic Phenotyping of Mutant Mice
25. Systemic First-Line Phenotyping 463
Index