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Macrophages and dendritic cells : methods and protocols / edited by Neil E. Reiner. — Totowa, N.J. : Humana, c2009. – (58.17/M592/v.531) |
Contents
Contents
Preface
Contributors
1 Generation of Retroviral Macrophage cDNA Expression Libraries and Functional Screening for Surface Receptors
2 Generation of Murine Growth Factor-Dependent Long-Term Dendritic Cell Lines to Investigate Host-Parasite Interactions
3 Macrophage Activation: Classical vs. Alternative
4 Analysis of Macrophage Phagocytosis: Quantitative Assays of Phagosome Formation and Maturation Using High-Throughput Fluorescence Microscopy
5 Detection of Activated Rab7 GTPase with an Immobilized RILP Probe
6 Probing In Vivo Origins of Mononuclear Phagocytes by Conditional Ablation and Reconstitution
7 Multivalent Protein Probes for the Identification and Characterization of Cognate Cellular Ligands for Myeloid Cell Surface Receptors
8 Exploiting Fc Chimaeric Proteins for the Identification of Ligands Specific for the Mannose Receptor 103
9 The Expression of Exogenous Genes in Macrophages: Obstacles and Opportunities 123
10 Genetic Modification of Murine Dendritic Cells by RNA Transfection 145
11 Recording Phagosome Maturation Through the Real-Time， Spectrofluorometric Measurement of Hydrolytic Activities 157
12 Derivation and Characterization of Murine Alternatively Activated (M2) Macrophages 173
13 Isolation and Culture of Human Hematopoietic Progenitors for Studies of Dendritic Cell Biology
14 Generation， Culture and Flow-Cytometric Characterization of Primary Mouse Macrophages
15 Analyzing Classical and Alternative Macrophage Activation in Macrophage/Neutrophil-Specific IL-4 Receptor-Alpha-Deficient Mice 225
16 Analysis of Matrix Metalloproteinase Secretion by Macrophages
17 Identification of Pathogen Receptors on Dendritic Cells to Understand Their Function and to Identify New Drug Targets 267
18 Stable Lentiviral Vector-Mediated Gene Silencing in Human Monocytic Cell Lines
19 Use of Fluorescent Probes to Detect Lipid Signaling Intermediates in Macrophages
20 Large-Scale Phagosome Preparation
21 Coordinate Regulation of Sphingosine Kinase and Actin Dynamics 347
Index
