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Bacteriophages : methods and protocols. volume 1, Isolation, characterization, and interactions / edited by Martha R.J. Clokie, Andrew M. Kropinski. — Totowa, N.J. : Humana, c2009. – (58.17/M592/v.501)

Contents

    Contents
    
    SECTION l. BACTEKIOPHAGE ISOLATION
    l Methods for the Isolation of Viruses from Environmental Samples
    2 Bacteriophage Enrichment from Water and Soil
    3 Isolation of Phage via Induction of Lysogens
    4 Isolation of Cyanophages from Aquatic Environments
    5 Isolation of Viruses from High Temperature Environments
    6 Isolation of Novel Large and Aggregating Bacteriophages
    SECTION 2. BACTERIOPHAGE CHARACTERIZATION
    7 Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay
    8 Enumeration of Bacteriophages by the Direct Platirv~ Plaque Assay
    9 Enumeration of Bacteriophages Using the Small Drop Plaque Assay System
    10 Determination of Virus Abundance by Epifluorescence Microscopy
    11 Enumeration of Bacteriophages Using Flow Cytometry
    12 Basic Phage Electron Microscopy
    13 Phage Classification and Characterization
    14 Phage Host Range and Efficiency of Plating
    15 Measurement of the Rate of Attachment of Bacteriophage to Cells
    16 Measurement of the Bacteriophage Inactivation Kinetics with Purified Receptors
    17 Bacteriophage Plaques: Theory and Analysis
    18 Practical Methods for Determining Phage Growth Parameters
    19 Phage Production and Maintenance of Stocks, Including Expected Stock Lifetimes
    SECTION 3. BACTERIOPHAGE-HOST INTERACTIONS20 Construction of Phage Mutants
    21 Modifying Bacteriophage k with Recombineering
    22 Identification and Isolation of Lysogens with Induced Prophage
    23 Generalized Transduction
    24 Preparation and Characterization of Anti-phage Serum
    25 Generalized Transduction by Lytic Bacteriophages
    Index 305