Bacteriophages : methods and protocols. volume 1, Isolation, characterization, and interactions / edited by Martha R.J. Clokie, Andrew M. Kropinski. — Totowa, N.J. : Humana, c2009. – (58.17/M592/v.501) |
Contents
Contents
SECTION l. BACTEKIOPHAGE ISOLATION
l Methods for the Isolation of Viruses from Environmental Samples
2 Bacteriophage Enrichment from Water and Soil
3 Isolation of Phage via Induction of Lysogens
4 Isolation of Cyanophages from Aquatic Environments
5 Isolation of Viruses from High Temperature Environments
6 Isolation of Novel Large and Aggregating Bacteriophages
SECTION 2. BACTERIOPHAGE CHARACTERIZATION
7 Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay
8 Enumeration of Bacteriophages by the Direct Platirv~ Plaque Assay
9 Enumeration of Bacteriophages Using the Small Drop Plaque Assay System
10 Determination of Virus Abundance by Epifluorescence Microscopy
11 Enumeration of Bacteriophages Using Flow Cytometry
12 Basic Phage Electron Microscopy
13 Phage Classification and Characterization
14 Phage Host Range and Efficiency of Plating
15 Measurement of the Rate of Attachment of Bacteriophage to Cells
16 Measurement of the Bacteriophage Inactivation Kinetics with Purified Receptors
17 Bacteriophage Plaques: Theory and Analysis
18 Practical Methods for Determining Phage Growth Parameters
19 Phage Production and Maintenance of Stocks, Including Expected Stock Lifetimes
SECTION 3. BACTERIOPHAGE-HOST INTERACTIONS20 Construction of Phage Mutants
21 Modifying Bacteriophage k with Recombineering
22 Identification and Isolation of Lysogens with Induced Prophage
23 Generalized Transduction
24 Preparation and Characterization of Anti-phage Serum
25 Generalized Transduction by Lytic Bacteriophages
Index 305