Proteases and cancer : methods and protocols / edited by Toni M. Antalis and Thomas H. Bugge. — [Totowa, N.J.] : Humana, c2009. – (58.17/M592/v.539) |
Contents
Contents
Preface
Contributors
1 Delineating Protease Functions During Cancer Development
2 Proteolytic Systems: Constructing Degradomes 33
3 Microarrays for Protease Detection in Tissues and Cells
4 Positional Scanning Synthetic Combinatorial Libraries for Substrate Profiling
5 Mixture-Based Peptide Libraries for Identifying Protease Cleavage Motifs
6 High Throughput Substrate Phage Display for Protease Profiling
7 Imaging Specific Cell Surface Protease Activity in Living Cells Using Keengineered Bacterial Cytotoxins
8 Cell-Based Identification of Natural Substrates and Cleavage Sites for Extracellular Proteases by SILAC Proteomics
9 Optical Proteolytic Beacons for In Vivo Detection of Matrix Metalloproteinase Activity
10 Dissecting the Urokinase Activation Pathway Using Urokinase-Activated Anthrax Toxin 175
11 On-Demand Cleavable Linkers for Radioimmunotherapy 191
Index