Expressed sequence tags (ESTs) : generation and analysis / edited by John Parkinson. — Totowa, N.J. : Humana, c2009. – (58.17/M592/v.533)

Contents

    Contents
    
    1 Expressed Sequence Tags: An Overview
    2 Strategies for Undertaking Expressed Sequence Tag (EST) Projects
    3 Construction ofcDNA Libraries: Focus on Protists and Fungi 33
    4 Generation of Full-Length eDNA Libraries: Focus on Plants
    5 Preparation of Full-Length eDNA Libraries: Focus on Metazoans 69
    6 Expressed Sequence Tags: Normalization and Subtraction of eDNA Libraries
    7 Generating EST Libraries: Trans-Spliced cDNAs
    8 Large-Scale Sequencing and Analytical Processing of ESTs
    9 EST Processing: From Trace to Sequence
    10 Obtaining Accurate Translations from Expressed Sequence Tags
    11 EST Databases and Web Tools for EST Projects 241
    12 Phylogenomic Analysis of EST Datasets
    13 Statistical Analysis of Expressed Sequence Tags
    14 Application of ESTs in Microarray Analysis
    15 Exploiting ESTs in Human Health