Nucleic acid and peptide aptamers : methods and protocols / edited by Gunter Mayer. — New York : Humana press, 2009. – (58.17/M592/v.535)

Contents

        Contents
    
    Preface
    Contributors
    PART I: NUCLEIC ACID APTAMERS
    1. Nucleic Acid Pool Preparation and Characterization
    2. In Vitro Selection of ssDNA Aptamers Using Biotinylated Target Proteins
    3. Isolating Aptamers Using Capillary Electrophoresis-SELEX (CE-SELEX) 33
    4. In Vitro Selection of Allosteric Ribozymes
    5. Cell-Specific Aptamers for Targeted Therapies
    6. Aptamers Targeting RNA Molecules
    7. Fluorescence Correlation Spectroscopy (FCS)-Based Characterisation of Aptamer Ligand Interaction
    8. Structural Probing Techniques on Natural Aptamers
    9. Determining Structures of RNA Aptamers and Riboswitches by X-Ray Crystallography
    10. Locked Nucleic Acid Aptamers
    11. Screening of Novel Inhibitors of HIV-1 Reverse Transcriptase with a Reporter Ribozyme Assay
    12. Aptamers as Artificial Gene Regulation Elements
    13. Aptamers and Biosensors 209
    14. Nanoparticles/Dip Stick
    15. In Vivo Imaging of Oligonucleotidic Aptamers
    PART II: PEPTIDE APTAMERS
    16. Selection of Peptides Interfering with Protein-Protein Interaction 263
    17. In Vitro Selection of Protein and Peptide Libraries Using mRNA Display
    18. Ligand-Regulated Peptide Aptamers
    19. Isolation of Peptide Aptamers to Target Protein Function
    20. Microbodies
    21. Peptide Aptamers for Small Molecule Drug Discovery 373
    22. Synthesis and Application of Peptides as Drug Carriers 389
    Index 405