siRNA and miRNA gene silencing : from bench to bedside / edited by Mouldy Sioud. — New York : Humana Press, c2009. – (58.17/M592/v.487) |
Contents
Contents
Preface
Contributors
1. Methods for Selecting Effective siRNA Sequences by Using Statistical and Clustering Techniques
2. Deciphering the Code of Innate Immunity Recognition of siRNAs...
3. Targeted Delivery of Antisense Oligonucleotides and siRNAs into Mammalian Cells
4. Local and Systemic Delivery of siRNAs for Oligonucleotide Therapy
5. Imaging of siRNA Delivery and Silencing
6. Recent Advances in Magnetofection and Its Potential to Deliver siRNAs in Vitro
7. In Vitro and In Vivo Gene Silencing by TransKingdom RNAi (tkRNAi) 147
8. Bacterial Delivery of siRNAs: A New Approach to Solid Tumor Therapy 161
9. The Therapeutic Potential of LNA-Modified siRNAs: Reduction of Off-Target Effects by Chemical Modification of the siRNA Sequence 189
10. pSM155 and pSM30 Vectors for miRNA and shRNA Expression 205
11. Targeting Oncogenes with siRNAs
12. Targeting Stromal-Cancer Cell Interactions with siRNAs
13. Therapeutic Potential of siRNA-Mediated Targeting of Urokinase Plasminogen Activator, Its Receptor, and Matrix Metalloproteinases 267
14. Silencing of HIF-1o~ by RNA Interference in Human Glioma Cells In Vitro and In Vivo 283
15. RNA Interference-Mediated Validation of Genes Involved in Telomere Maintenance and Evasion of Apoptosis as Cancer Therapeutic Targets 303
16. Treating Respiratory Viral Diseases with Chemically Modified, Second Generation Intranasal siRNAs 331
17. Progress in the Therapeutic Applications of siRNAs Against HIV-1 343
18. Protein Components of the microRNA Pathway and Human Diseases 369
19. Intron-Mediated RNA Interference and microRNA Biogenesis
20. Emergence of a Complex Relationship Between HIV-1 and the microRNA Pathway 415
21. Synthetic microRNA Targeting Glioma-Associated Antigen-1 Protein 435
22. Therapeutic Targeting of Gene Expression by siRNAs Directed Against BCR-ABL Transcripts in a Patient with Imatinib-Resistant Chronic Myeloid Leukemia 451