Potassium channels : methods and protocols / edited by Jonathan D. Lippiat. — New York : Humana Press, c2008. – (58.17/M592/v.491)

Contents

    Contents
    
    Prcfacc
    Contributors
    PART I. METHODS FOR STUDYING POTASSIUM CHANNEL TRANSCRIPTION AND MRNA SPLICING
    1. Identifying Transcriptional Regulatory Regions Using Reporter Genes and DNA-Protein Interactions by Chromatin Immunoprecipitation.
    2. Quantitative RT-PCR Methods for Investigation of Low Copy Potassium Channel Gene Expression in Native Murine Arteries
    3. Cloning of Potassium Channel Splice Variants from Tissues and Cells
    PART II. METHODS FOR STUDYING THE CELL BIOLOGY OF POTASSIUM CHANNEL PROTEINS
    4. Chemiluminescence Assays to Investigate Membrane Expression and Clathrin-Mediated Endocytosis of KATv Channels
    5. Investigation of KATe Channel Endocytosis by Immunofluorescence
    6. Investigation of KATv Channel Endocytosis and Cell Surface Density by Biotinylation and Western Blotting
    7. Lipid Microdomains and K+ Channel Compartmentation: Detergent and Non-Detergent-Based Methods for the Isolation and Characterisation of Cholesterol-Enriched Lipid Rafts
    8. Determination of Phosphoinositide Binding to K+ Channel Subunits Using a Protein-Lipid Overlay Assay
    9. Protein Complex Analysis of Native Brain Potassium Channels by Proteomics
    PART III. ELECTROPHYSIOLOGICAL TECHNIQUES FOR THE STUDY OF POTASSIUM CHANNEL FUNCTION
    10. Xenopus Oocytes as a Heterologous Expression System for Studying Ion Channels with the Patch-Clamp Technique
    11. Whole-Cell Recording Using the Perforated Patch Clamp Technique
    12. Recording the Activity of ATP Cell-Attached Configuration.
    13. Planar Patch Clamp: Advances in Electrophysiology
    14. Analysing Steroid Modulation of BIQa Channels Reconstituted into Planar Lipid Bilayers
    PART IV. OPTICAL TECHNIQUES FOR THE STUDY OF POTASSIUM CHANNEL FUNCTION AND INTERACTIONS
    15. Using Bioluminescence Resonance Energy Transfer to Measure Ion Channel Assembly
    16. The Use of FRET Microscopy to Conformational Rearrangements with the GIRK Channels
    17. The Voltage-Clamp Fluorometry Technique
    PART V. METHODS FOR STUDYING POTASSIUM CHANNELOPATHIES AND PHARMACOLOGICAL MODULATORS
    18. Identification of Mutations in the Kir6.2 Subunit of the KATp Channel
    19. Modulation of Potassium Ion Channel Proteins Utilising Antibodies.
    20. Fluorescence-Based Tl+ -Influx Assays as a Novel Approach for Characterization of Small-Conductance Ca2+-Activated K+ Channel Modulators
    21. Rubidium Efflux as a Tool for the Pharmacological Characterisation of Compounds with BK Channel Opening Properties
    22. Recording hERG Potassium Currents and Assessing the Effects of Compounds Using the Whole-Cell Patch-Clamp Technique
    Index