Auditory and vestibular research : methods and protocols / edited by Bernd Sokolowski. — New York : Humana Press, c2009. – (58.17/M592/v.493)

Contents

    Contents
    
    Preface
    Contributors
    PART I. NUCLEIC ACID PROTOCOLS
    1 RNA Isolation from Xenopus Inner Ear Sensory Endorgans for Transcriptional Profiling and Molecular Cloning
    2 Synthesis of Biotin-Labeled RNA for Gene Expression Measurements Using oligonucleotide Arrays
    3 In situ Hybridization Approach to Study mRNA Expression and Distribution in Cochlear Frozen Sections
    4 Lineage Analysis of Inner Ear Cells Using Genomic Tags for Clonal Identification
    5 Genetic Fate-Mapping Approaches: New Means to Explore the Embryonic Origins of the Cochlear Nucleus
    6 The Practical Use of Cre and loxP Technologies in Mouse Auditory Research
    7 Helios Gene Gun-Mediated Transfection of the Inner Ear Sensory Epithelium
    8 Electroporation-Mediated Gene Transfer to the Developing Mouse Inner Ear
    9 Isolation of Sphere-Forming Stem Cells from the Mouse Inner Ear
    10 Molecular Biology of Vestibular Schwannomas
    11 Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis of Otitis Media Causing Pathogens
    12 Fluorescence "In Situ" Hybridization for the Detection of Biofilm in the Middle Ear and Upper Respiratory Tract Mucosa
    13 Positional Cloning of Deafness Genes
    PART II. AMINO ACID PROTOCOLS
    14 Twist-Off Purification of Hair Bundles
    15 Yeast Two-Hybrid Screening to Test for Protein-Protein Interactions in the Auditory System
    16 The Use of 2-D Gels to Identify Novel Protein-Protein Interactions in the Cochlea
    17 Identification of functionally important residues/domains in membrane proteins using an evolutionary approach coupled with systematic mutational analysis
    18 In Vivo Verification of Protein Interactions in the Inner Ear by Coimmunoprecipitation
    19 Identification of Transcription Factor-DNA Interactions Using Chromatin Immunoprecipitation Assays
    20 Surface Plasmon Resonance (SPR) Analysis of Binding Interactions of Proteins in Inner-Ear Sensory Epithelia
    21 Multiplexed isobaric tagging protocols for quantitative mass spectrometry approaches to auditory research
    PART III. IMAGING PROTOCOLS
    22 Fluorescence Microscopy Methods in the Study of Protein Structure and Function
    23 Ion Imaging in the Cochlear Hair Cells
    24 Atomic Force Microscopy in Studies of the Cochlea
    Index