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Mass spectrometry of proteins and peptides : methods and protocols / edited by Mary S. Lipton and Ljiljana Pasa-Tolic. — 2nd ed. — New York : Humana Press, c2009. – (58.17/M592/v.492) |
Contents
Contents
Preface
Contributors
1. Generation and Analysis of Multidimensional Protein Identification Technology Datasets 1
2. Quantitative Peptide and Protein Profiling by Mass Spectrometry 21
3. Label-Free Relative Quantitation of Prokaryotic Proteomes Using the Accurate Mass and Time Tag Approach 39
4. Classical Proteomics: Two-Dimensional Electrophoresis/MALDI Mass Spectrometry
5. The Use of Difference In-Gel Electrophoresis for Quantitation of Protein Expression
6. Liquid-Chromatography-Mass Spectrometry of Thylakoid Membrane Proteins 113
7. High Accuracy Mass Spectrometry in Large-Scale Analysis of Protein Phosphorylation 131
8. Manual Validation of Peptide Sequence and Sites of Tyrosine Phosphorylation from MS/MS Spectra 143
9. Assigning Glycosylation Sites and Microheterogeneities in Glycoproteins by Liquid Chromatography/Tandem Mass Spectrometry 161
10. Structure Analysis of N -Glycoproteins 181
11. Capillary Zone Electrophoresis-Mass Spectrometry for the Characterization of Isoforms of Intact Glycoproteins 201
12. Top-Down Proteomics on a High-field Fourier Transform Ion Cyclotron Resonance Mass Spectrometer 215
13. Capillary Isoelectric Focusing/Reversed Phase Liquid Chromatography/Mass Spectrometry
14. Integrating Accelerated Tryptic Digestion into Proteomics Workflows 241
15. Hydrogen/Deuterium Exchange Mass Spectrometry 255
16. Mass Spectrometry Detection and Characterization of Noncovalent Protein Complexes
17. Chemical Cross-Linking for Protein-Protein Interaction Studies 283
18. Tissue Analysis with High-Resolution Imaging Mass Spectrometry 295
19. Proteomic Global Profiling for Cancer Biomarker Discovery 309
20. Analysis of Protein Glycosylation and Phosphorylation Using Liquid Phase Separation， Protein Microarray Technology， and Mass Spectrometry 321
21. Transthyretin Mass Determination for Detection of Transthyretin Familial Amyloid 353
22. Characterization of Microorganisms by MALDI Mass Spectrometry 367
23. Mass Spectrometric Characterization of Neuropeptides 381
24. Peptide and Protein Ion/Ion Reactions in Electrodynamic Ion Traps: Tools and Methods 395
25. Electron Capture Dissociation LC/MS/MS for Bottom-Up Proteomics 413
26. Two-Dimensional Ion Mobility Analyses of Proteins and Peptides 417
27. Proteomics for Validation of Automated Gene Model Predictions 447
28. Support Vector Machines for Improved Peptide Identification from Tandem Mass Spectrometry Database Search
Index 461
