 |
Genetic modification of hematopoietic stem cells : Methods and protocols / edited by Christopher Baum. — New York : Humana Press, c2009. – (58.17/M592/v.506) |
Contents
Contents
Preface
Contributors
1. Immunomagnetic Enrichment of Human and Mouse Hematopoietic Stem Cells for Gene Therapy Applications
2. Isolation of Human and Mouse Hematopoietic Stem Cells
3. Murine Hematopoictic Stem Cell Transduction Using Retroviral Vectors
4. Genetic Modification of Human Hematopoietic Cells Preclinical Optimization of Oncoretroviral-mediated Gene Transfer for Clinical Trials
5. Short-Term Culture of Human CD34+ Cells for Lentiviral Gene Transfer
6. T Cell Culture for Gammaretroviral Transfer
7. Retroviral Transduction of Murine Primary T Lymphocytes
8. Lentiviral Vector Gene Transfer into Human T Cells
9. DNA Transposons for Modification of Human Primary T Lymphocytes 115
10. Retroviral Gene Transfer into Primary Human Natural Killer Cells 127
11. Lentiviral Vector-Mediated Genetic Programming of Mouse and Human Dendritic Cells
12. In Situ (In Vivo) Gene Transfer into Murine Bone Marrow Stem Cells
13. In Vivo and Ex Vivo Gene Transfer in Thymocytes and Thymocyte Precursors
14. Design and Production of Retro- and Lentiviral Vectors for Gene Expression in Hematopoietic Cells
15. Knock-Down of Gene Expression in Hematopoietic Cells
16. The Use of Retroviral Vectors for tet-Regulated Gene Expression in Cell Populations 221
17. Detection of Replication Competent Retrovirus and Lentivirus 243
18. Release Testing of Retroviral Vectors and Gene-Modified Cells 265
19. Copy Number Determination of Genetically-Modified Hematopoietic Stem Cells
20. Tissue Procurement for Molecular Studies Using Laser-Assisted Microdissection
21. Leukemia Diagnosis in Murine Bone Marrow Transplantation Models 311
22. Humanized Mouse Models to Study the Human Haematopoietic Stem Cell Compartment
23. Canine Models of Gene-Modified Hematopoiesis
24. Detection of Retroviral Integration Sites by Linear Amplification-Mediated PCR and Tracking of Individual Integration Clones in Different Samples 363
25. Retroviral Insertion Site Analysis in Dominant Haematopoietic Clones 373
26. Tracking Gene-Modified T Cells In Vivo
27. DNA Microarray Studies of Hematopoietic Subpopulations
28. Quantification of Genomic Mutations in Murine Hematopoietic Cells 423
29. Proteomics Studies After Hematopoietic Stem Cell Transplantation 437
30. Spectral Karyotyping and Fluorescence In Situ Hybridization of Murine Cells
31. Database Setup for Preclinical Studies of Gene-Modified Hematopoiesis
32. The US and EU Regulatory Perspectives on the Clinical Use of Hematopoietic Stem/Progenitor Cells Genetically Modified Ex Vivo by Retroviral Vectors
Index 489
