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Proteomics of the nervous system / edited by Hans Gerd Nothwang and Steven E. Pfeiffer. — Weinheim : Wiley-VCH Verlag GmbH &co. KGaA, c2008. – (58.17421/P967pn) |
Contents
Contents
Preface XIII
Abbreviations XV
List of Contributors XXI
Colour Plates XXV
1 Protein Purification 1
1.1 Introduction 1
1.2 Types of Molecular Interactions and Variables that Affect Them 1
1.3 Protein Properties that can be Used as Handles for Purification 4
1.4 Types of Separation Methods 10
1.5 Protein Inactivation and How to Prevent It 10
1.6 Protein Purification Strategy 11
1.7 Overproducing Recombinant Proteins 13
1.8 Refolding Proteins Solubilized from Inclusion Bodies 15
2 Common Methods in Proteomics 19
2.1 Introduction 19
2.2 Protein Separation by Polyacrylamide Gel Electrophoresis 19
2.3 Liquid Chromatography-Based Separation Techniques 23
2.4 Mass Spectrometry and Data Analysis 26
2.5 Protein Quantification Methods 29
2.6 Summary 31
3 Proteomic Technologies for the Analysis of Protein Complexes 35
3.1 Introduction 35
3.2 Methods for Isolating Protein Complexes 36
3.3 Techniques for Identifying Protein Complexes 40
3.4 Yeast Two-Hybrid Screening 44
3.5 Fluorescence Microscopy 47
3.6 Conclusions 50
4 Analysis of Membrane Protein Complexes by Blue N ative- PAG E 55
4.1 Introduction 55
4.2 Protocols 62
4.3 Outlook 66
5 Current Approaches to Protein Structure Analysis 71
5.1 Bioinformatics 71
5.2 Protein Production 75
5.3 Purification 78
5.4 Structure Determination by X-Ray Crystallography 79
5.5 Structure-Based Drug Design 83
5.6 Structure Determination by NMR 84
6 Primer to Proteins Underlying Cellular Complexity and Function in the Nervous System 91
6.1 Molecular Complexity in Neurons 91
6.2 Cellular Complexity in the Nervous System and Neuronal Cell Types 100
6.3 Conclusion 109
7 Proteome Analysis of Plasma Membranes 123
7.1 Introduction 123
7.2 Protocols 131
7.3 Outlook 135
8 Proteome Analysis of Synaptic Vesicles 141
8.1 Introduction 141
8.2 Protocols 150
8.3 Summary and Outlook 162
9 Proteomics Analysis of the Synapse 167
9.1 Introduction 167
9.2 Protocols 172
9.3 Outlook 175
10 Peptidomics of the Nervous System 179
10.1 Introduction 179
10.2 Protocols 182
10.3 Outlook 187
11 Analysis of Posttranslational Modifications 189
11.1 Introduction 189
11.2 Protocols 197
11.3 Outlook 202
12 Proteomics of" Neurodegenerative Disorders 209
12.1 Introduction 209
12.2 History of Brain Proteomics 210
12.3 Alzheimer's Disease 211
12.4 Parkinson's Disease 215
12.5 Administrative Realization of Neuroproteomics: The Brain Proteome Projects 221
13 The Search for Protein Biomarkers of Neurobiological Diseases 229
13.1 Introduction 229
13.2 Methodology 237
13.3 Outlook 241
14 Redox Proteomics: Applications to Age-Related Neurodegenerative Disorders 255
14.1 Introduction 255
14.2 Methods 263
14.3 Future Directions 266
15 Paranode Structure and Function 271
15.1 Introduction 271
15.2 Protocols 276
15.3 Outlook 278
