Lipid signaling protocols / edited by Banafshe Larijani, Rudiger Woscholski, Colin A. Rosser. — New York : Humana Press, c2009. – (58.17/M592/v.462)

Contents

    Contents
    
    Part I Lipid Systems and Techniques
    1 Eicosanoids: Generation and Detection in Mammalian Cells
    2 Lipidomic Analysis of Phospholipids and Related Structures by Liquid Chromatography-Mass Spectrometry
    3 Measurement of Polyphosphoinositides in Cultured Mammalian Cells Frank T. Cooke
    4 Inositol Lipid-Dependent Functions in Saccharomyces cerevisiae: Analysis of Phosphatidylinositol Phosphates 59
    5 Methods for the Determination of the Mass of Nuclear Ptdlns4P， PtdIns5P， and Ptdlns(4，5)P2
    6 Lipid Quantification and Structure Determination of Nuclear Envelope Precursor Membranes in the Sea Urchin 89
    7 Solution and Solid-State NMR of Lipids
    8 Spatial Localization of PtdlnsP2 in Phase-Separated Giant Unilamellar Vesicles with a Fluorescent PLC-delta 1 PH Domain 135
    9 Studying Cell-to-Cell Interactions: An Easy Method of Tethering Ligands on Artificial Membranes
    10 Ceramide-Induced Transbilayer (Flip-Flop) Lipid Movement in Membranes
    11 Effects of Sterols on the Development and Aging of Caenorhabditis elegans
    12 Proteomic Analysis of the Sterol-Mediated Signaling Pathway in Caenorhabditis elegans
    Part II Lipid Metabolism and Recognition
    13 Studying the Subcellular Localization and DNA-Binding Activity of FoxO Transcription Factors， Downstream Effectors of PI3K/Akt 201
    14 Measurement of PTEN Activity in vivo by Imaging Phosphorylated Akt
    15 Analysis of Phosphatidylinositol 3，4，5 Trisphosphate 5-Phosphatase Activity by in vitro and in vivo Assays
    16 Measuring Phospholipase D Activity in Insulin-Secreting Pancreatic β-Cells and Insulin-Responsive Muscle Cells and Adipocytes 241
    17 Protein Kinase C as an Effector of Lipid-Derived Second Messengers 253
    18 Detection of Myotubularin Phosphatases Activity on Phosphoinositides in vitro and ex vivo 265
    19 Quantification of Multiple Phosphatidylinositol 4-Kinase Isozyme Activities in Cell Extracts
    20 Phospholipid-Interacting Proteins by Solution-State NMR Spectroscopy 291
    21 Revealing Signaling in Single Cells by Single- and Two-Photon Fluorescence Lifetime Imaging Microscopy 307
    22 Phosphoinositide (PI) 3-Kinase Assays
    23 Measurement of Phosphatidylinositol and Phosphatidylcholine Binding and Transfer Activity of the Lipid Transport Protein PITP
    24 In vitro Reconstitution of Activation of PLC by Ras and Rho GTPases 379
    25 Assaying Endogenous Phosphatidylinositol-4-Phosphate 5-Kinase (PIP5K) Activities 391
    26 Preparation of Membrane Rafts
    Index