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Plant signal transduction : methods and protocols / edited by Thomas Pfannschmidt. — New York : Humana Press, c2009. – (58.17/M592/v.479) |
Contents
Contents
Preface
Contributors
1 Multiple Dimensions in Plant Signal Transduction: An Overview 1
2 Phenotyping of Arabidopsis Mutants for Developmental Effects of Gene Deletions
3 Phenotyping of Abiotic Responses and Hormone Treatments in Arabidopsis 35
4 Accurate Real-time Reverse Transcription Quantitative PCR 61
5 Probing Spatio-temporal Intracellular Calcium Variations in Plants 79
6 Dynamic Redox Measurements with Redox-Sensitive GFP in Plants by Confocal Laser Scanning Microscopy 93
7 Imaging of Reactive Oxygen Species in vivo
8 Identification of Thioredoxin Targeted Proteins Using Thioredoxin Single Cysteine Mutant-immobilized Resin 117
9 Determination of in vivo Protein Phosphorylation in Photosynthetic Membranes
10 Examining Protein Stability and Its Relevance for Plant Growth and Development
11 Probing Circadian Rhythms in Chlamydomonas rheinhardtii by Functional Proteomics 173
12 Bimolecular Fluorescence Complementation (BiFC) to Study Protein-protein Interactions in Living Plant Cells 189
13 Fluorescence Cross-Correlation Spectroscopy of Plant Proteins 203
14 The Determination of Protein-protein Interactions by the Mating-based Split-ubiquitin-system (mbSUS)
15 Functional Complementation of Yeast Mutants to Study Plant Signalling Pathways 235
16 Phosphatase Activities Analyzed by in vivo Expressions 247
17 Chromatin Immunoprecipitation Experiments to Investigate in vivo Binding of Arabidopsis Transcription Factors to Target Sequences 261
18 Fluorescence-based Electrophoretic Mobility Shift Assay in the Analysis of DNA-binding Proteins 273
19 Analysis of Plant Regulatory DNA sequences by the Yeast-One-Hybrid Assay
20 Analysis of Plant Regulatory DNA Sequences by Transient Protoplast Assays and Computer Aided Sequence Evaluation 311
21 RNA-protein Interaction Mediating Post-transcriptional Regulation in the Circadian System 337
