Wnt signaling. v. 2, Pathway models / edited by Elizabeth Vincan. — New York, N.Y. : Humana Press, c2008. – (58.17/M592/v.469)

Contents

    Contents
    
    Contributors
    Contents of Volume I
    PART I: INTRODUCTION
    1. Evolution of the Wnt Pathways
    PART II: DICTYOSTELIUM
    2. Dictyostelium Development: A Prototypic Wnt Pathway?
    3. Monitoring Patterns of Gene Expression in Dictyostelium by β-galactosidase Staining
    4. Use of the Dictyostelium Stalk Cell Assay to Monitor GSK-3 Regulation
    PART III: CNIDARIANS
    5. Wnt Signaling in Cnidarians
    6. Detecting Expression Patterns of Wnt Pathway Components in Nematostella vectensis Embryos 55
    7. Detection of Expression Patterns in Hydra Pattern Formation
    PART IV: C. ELEGANS
    8. Analysis of Wnt Signaling During Caenorhabditis elegans Postembryonic Development
    9. Wnt Signaling During Caenorhabditis elegans Embryonic Development
    PART V: DROSOPHILA
    10. Function of the Wingless Signaling Pathway in Drosophila
    11. Visualization of PCP Defects in the Eye and Wing of Drosophila melanogaster
    12. Wingless Signaling in Drosophila Eye Development
    13. High-Throughput RNAi Screen in Drosophila
    PART VI: SEA URCHIN
    14. Wnt Signaling in the Early Sea Urchin Embryo
    16. Functional Analysis of Wnt Signaling in the Early Sea Urchin Embryo Using mRNA Microinjection
    PART VII: ZEBRAFISH
    17. Wnt Signaling Mediates Diverse Developmental Processes in Zebrafish 225
    18. Determination of mRNA and Protein Expression Patterns in Zebrafish 253
    20. Neural Patterning and CNS Functions of Wnt in Zcbrafish 301
    PART VIII: XENOPUS
    21. Studying Wnt Signaling in Xenopus
    SECTION A: METHODS FOR STUDYING WNT SIGNALING IN XENOPUS EMBRYOS
    22. Analysis of Gene Expression in Xenopus Embryos
    23. Detection of Nuclear ~-catenin in Xenopus Embryos
    24. Transgenic Reporter Tools Tracing Endogenous Canonical Wnt Signaling in Xenopus
    25. Gain-of-Function and Loss-of-Function Strategies in Xenopus
    26. How the Mother Can Help: Studying Maternal Wnt Signaling by Anti-sense-mediated Depletion of Maternal mRNAs and the Host Transfer Technique
    27. Inducible Gene Expression in Transient Transgenic Xenopus Embryos
    28. Wnt-Frizzled Interactions in Xenopus
    SECTION B: WNT SIGNALING FUNCTION IN XENOPUS DEVELOPMENT
    29. Dorsal Axis Duplication as a Functional Readout for Wnt Activity
    30. Regulation of Convergent Extension by Non-canonical Wnt Signaling in the Xenopus Embryo 477
    31. Frizzled-7-dependent Tissue Separation in the Xenopus Gastrula 485