Redox-mediated signal transduction : methods and protocols / edited by John T. Hancock. — New York, NY : Humana Press, c2008. – (58.17/M592/v.476)

Contents

        Contents
    
    Preface
    Contributors
    1 The Role of Redox in Signal Transduction
    2 The Measurement of Nitric Oxide and its Metabolites in Biological Samples by Ozone-based Chemiluminescence
    3 Detection and Measurement of Reactive Oxygen Intermediates in Mitochondria and Cells
    4 Redox-sensitive Green Fluorescent Protein: Probes for Dynamic Intracellular Redox Responses. A Review
    5 Measuring Redox Changes in Vivo in Leaves: and Technical Challenges
    6 Imaging of Intracellular Hydrogen Peroxide Production with Hyper Upon Stimulation of Hela Cells with Egf . .
    7 Tools to Investigate ROS Sensitive Signalling Proteins 87
    8 Methods for Preparing Crystals of Reversibly Oxidized Proteins: Crystallization of Protein Tyrosine Phosphatase lb as an Example 101
    9 Methods for the Study of Redox-mediated Changes in p53 Structure and Function
    10 Redox Regulation and Trapping Sulphenic Acid in the Peroxide Sensitive Human Mitochondrial Branched Chain Aminotransferase 139
    11 Detection of Carbonylated Proteins in 2-D SDS Page Separations 153
    12 Analysis of Global and Specific Changes in the Disulfide Proteome Using Redox 2D-PAGE 165
    13 Protein-thiol Oxidation， from Single Proteins to Proteome-wide Analyses 181
    14 Analysis of Redox Relationships in the Plant Cell Cycle: Determinations of Ascorbate， Glutathione and Poly (ADPribose) Polymerase (PARP) in Plant Cell Cultures
    15 Generation and Detection of S-Nitrosothiols