Advanced protocols in oxidative stress. v. 1 / edited by Donald Armstrong. — New York, N.Y. : Humana Press, c2008. – (58.17/M592/v.477)

Contents

        Contents
    
    Preface
    Contributors
    Part I: REACTIVE OXYGEN AND NITROGEN TECHNIQUES
    1. Novel Designed Probes for the Characterization of Oxidative Stress in Biological Fluids， Cells， and Tissues
    2. A Rapid and Selective Mass Spectrometric Method for the Identification of Nitrated Proteins
    3. An Easy and Reliable Automated Method to Estimate Oxidative Stress in the Clinical Setting
    4. Correlative Transmission Microscopy: Cytochemical Localization and Immunocytochemical Localization in Studies of Oxidative and Nitrosative Stress
    5. Detection of Specifically Oxidized Apolipoproteins in Oxidized HDL
    6. High Performance Liquid Chromatography/Electron Spin Resonance/Mass Spectrometry Analyses of Lipid-Derived Radicals
    7. EPR Spin-Trapping and Nano LC MS/MS Techniques fbr DEPMPO/'OOH and Immunospin-Trapping with Anti-DMPO Antibody in Mitochondrial Electron Transfer System
    8. Determination of High Mitochondrial Membrane Potential in Spermatozoa Loaded with the Mitochondrial Probe 5， 5'，6，6'-Tetrachloro- 1，1'，3，3'- Tetraethylbenzimidazolyl-Carbocyanine Iodide (JC-1) and for Using Fluorescence-Activated Flow Cytometry
    9. 2，2，6，6-Tetramethylpiperidin-l-Oxyl Probes for Evaluating Oxidative Stress on the Cell Membrane and Mitochondria
    10. Zymographical Techniques for Detection of Matrix Mctalloproteinases
    11. The Use of In Vivo Microdialysis Techniques to Detect Extracellular ROS in Resting and Contracting Skeletal Muscle
    12. Cell-Free Antibody Capture Method fbr Analysis of Detergent-Resistant Membrane Rafts
    13. Determination of Acrolein by High-Voltage Capillary Electrophoresis from Oxidized Fatty Acids
    Part II: ANTIOXIDANT TECHNOLOGY AND APPLICATION
    14. Cupric Ion Reducing Antioxidant Capacity Assay for Food Antioxidants: Vitamins， Polyphenolics， and Flavonoids in Food Extracts
    15. Redox Property of Ribonucleotide Reductase Small Subunit M2 and p53R2
    16. Antioxidant QSAR Modeling as Exemplified on Polyphenols
    17. Immunohistochemical Staining of Cyclooxygenases with Monoclonal Antibodies
    18. Examining the Endogenous Antioxidant Response Through Immunofluorescent Analysis of Nrf2 in Tissue
    19. Determination of Oxidizcd and Reduced CoQ10 and CoQ9 in Human Plasma/Serum Using HPLC-ECD
    20. Paraoxonases (PON1， PON2， PON3) Analysis In Vitro and In Vivo in Relation to Cardiovascular Diseases
    21. Preparation， Characterization， and Use of Antioxidant-Liposomes
    22. Antioxidant Activity of Biotransformed Sex Hormones Facilitated by Bacillus stearothermophilus
    23. Biolistic Mediatcd Production of Transgenic Oil Palm
    24. Preparation of a Multi-antioxidant Formulation
    Part III: GENE EXPRESSION
    25. A Functional Genomics Approach to Identify and Characterize Oxidation Resistance Genes
    26. Genome-Wide Overexpression Screen for Activators ofAntioxidant Gene Transcription
    27. Method for Conducting Microarray Study of Oxidative Stress Induced Gene Expression
    28. A Systems Approach Demonstrating Sphingolipid-Dependent Transcription in Stress Responses
    29. Bioluminescence: Imaging Modality fbr In Vitro and In Vivo Gene Expression
    Part IV: STEM CELLS
    30. Reactive Oxygen Species and Upregulation of NADPH Oxidases in Mechanotransduction of Embryonic Stem Cells
    Part V: BIOSTATISTICS
    31. Pooling Data When Analyzing Biomarkers Subject to a Limit of Detection