Molecular biomethods handbook / edited by John M. Walker, Ralph Rapley. — 2nd ed. — Totowa, NJ : Humana Press, c2008. – (58.178073/M718/2nd ed.)

Contents

    Content
    
    Part A Nucleic Acid Methods
    1 The Manipulation of Nucleic Acids: Basic Tools and Techniques
    2 Restriction Enzymes: Tools in Clinical Research
    3 Principles and Medical Applications of the Polymerase Chain Reaction
    4 Probe Design， Production， and Applications
    5 Southern Blotting as a Diagnostic Method
    6 Capillary Electrophoresis of DNA: Biomedical Applications
    7 Denaturing High-Performance Liquid Chromatography(DHPLC) for Nucleic Acid Analysis 89
    8 Denaturing Gradient Gel Electrophoresis (DGGE) 107
    9 Single Strand Conformation Polymorphism (SSCP) Analysis 117
    10 Randomly Amplified Polymorphic DNA (RAPD): A Useful Tool for Genomic Characterization of Different Organisms 133
    11 Quantification of mRNA Using Real Time RT-PCR: The SYBR Solution 149
    12 Quantitative Analysis of DNA Sequences by PCR and Solid-Phase Minisequencing
    13 Multiplex Amplifiable Probe Hybridization (MAPH) 179
    14 Gene Expression Profiling
    15 Comparative Genomic Hybridization in Clinical and Medical Research 213
    16 Subtractive Hybridization 227
    17 Fluorescence In Situ Hybridization 239
    18 Quantitative Trait Locus Mapping to Identify Genes for Complex Traits in Mice 257
    19 cDNA Microarrays 269
    20 Mapping Techniques 291
    21 Single Nucleotide Polymorphisms 311
    22 Gene/Protein Sequence Analysis: A Compilation of Bioinformatic Tools 323
    Part B Protein and Cell Methods
    23 Protein Electrophoresis 351
    24 Protein Blotting 365
    25 Capillary Electrophoresis of Proteins 377
    26 Autoradiography and Fluorography 397
    27 Mass Spectrometry of Proteins and Peptides 411
    28 Post-Translational Modifications of Proteins 427
    29 Protein Microarray Technology 451
    30 Protein-Protein Interactions 463
    31 Glycoprotein Analysis 495
    32 Solid-Phase Peptide Synthesis 515
    33 Monoclonal Antibodies 547
    34 Antibody Phage Display 563
    35 Protein Engineering 587
    36 Directed Protein Evolution 631
    37 Enzyme Linked Immunosorbent Assay (ELISA) 657
    38 Epitope Mapping: Identification of Antibody-Binding Sites on Protein Antigens 683
    39 Quantum Dots 697
    40 Ion-Exchange Chromatography 711
    41 Size-Exclusion Chromatography 719
    42 Hydrophobic Interaction Chromatography 731
    43 Affinity Chromatography 741
    44 High Performance Liquid Chromatography(HPLC) of Peptides and Proteins 767
    45 Amino Acid Analysis 793
    46 Surface Plasmon Resonance 809
    47 Macromolecular Crystallography 821
    48 Microchip Devices for Bioanalysis 851
    49 Mammalian Cell Culture 861
    50 Plant Tissue Culture 875
    51 Stem Cells and Regenerative Medicine 905
    52 Cryopreservation: Conservation of Bioresources at Ultra Low Temperatures 917
    53 Magnetic Resonance Imaging
    54 Electron Microscopy
    55 Confocal Microscopy
    56 Laser Microdissection 1027
    57 Biomedical Uses of Flow Cytometry 1039
    58 Immunomicroscopy
    59 In Situ Hybridization
    60 High Throughput Screening 1097
    Index 1119