Methods in cell biology. v. 88, Introduction to electron microscopy for biologists / edited by Terence D. Allen. — Amsterdam : Elsevier, c2008. – (58.1574/M592/v.88)

Contents

    CONTENTS
    
    PART I Exploring the Organisation of the Cell by Electron Microscopy
    SECTION 1 Basic Transmission and Scanning Electron Microscopy
    1. High Pressure Freezing and Freeze Substitution of Schizosaccharomyces pombe and Saccharomyces cerevisiae for TEM
    2. Electron Probe X-ray Microanalysis for the Study of Cell Physiology
    3. Preparation of Cells and Tissues for Immuno EM
    4. Quantification of Structures and Gold Labeling in Transmission Electron Microscopy
    5. Combined Video Fluorescence and 3D Electron Microscopy
    6. From Live-Cell Imaging to Scanning Electron Microscopy (SEM): The Use of Green Fluorescent Protein (GFP) as a Common Label
    7. Immunolabeling for Scanning Electron Microscopy (SEM) and Field Emission SEM
    8. Immunogold Labeling of Thawed Cryosections
    9. Close-to-Native Ultrastructural Preservation by High Pressure Freezing
    10. High-Pressure Freezing and Low-Temperature Fixation of Cell Monolayers Grown on Sapphire Coverslips
    11. Freeze-Fracture Cytochemistry in Cell Biology
    PART II Electron Microscopy of Specific Cellular Structure
    SECTION 1 The Cell Membrane
    12. Three-Dimensional Molecular Architecture of the Plasma-Membrane-Associated Cytoskeleton as Reconstructed by Freeze-Etch Electron Tomography
    13. Visualization of Dynamins
    SECTION 2 The Cytoskeleton
    14. Correlated Light and Electron Microscopy of the Cytoskeleton
    15. Electron Microscopy of Intermediate Filaments: Teaming up with Atomic Force and Confocal Laser Scanning Microscopy
    16. Studying Microtubules by Electron Microscopy
    SECTION 3 Extracellular Matrix and Cell Junctions
    17. Electron Microscopy of Collagen Fibril Structure In Vitro and In Vivo Including Three-Dimensional Reconstruction
    18. Visualization of Desmosomes in the Electron Microscope
    SECTION 4 The Nucleus
    19. A Protocol for Isolation and Visualization of Yeast Nuclei by Scanning Electron Microscopy
    20. Scanning Electron Microscopy of Nuclear Structure
    21. Electron Microscopy of Lamin and the Nuclear Lamina in Caenorhabditis elegans
    22. Visualization of Nuclear Organization by Ultrastructural Cytochemistry
    23. Scanning Electron Microscopy of Chromosomes
    PART III Cells and Infectious Agents
    24. Infection at the Cellular Level
    25. Electron Microscopy of Viruses and Virus-Cell Interactions