Methods in cell biology. v. 83, Cell mechanics / edited by Yu-Li Wang, Dennis E. Discher. — Amsterdam : Elsevier, c2007. – (58.1574/M592/v.83)

Contents

    CONTENTS
    
    Preface
    PART l Basic Concept and Preparation Culture Substrates for Cell Mechanical Studies
    1. Basic Rheology for Biologists
    2. Polyacrylamide Hydrogels for Cell Mechanics: Steps Toward Optimization and Alternative Uses
    3. Microscopic Methods for Measuring the Elasticity of Gel Substrates for Cell Culture: Microspheres， Microindenters， and Atomic Force Microscopy
    4. Surface Patterning
    5. Molecular Engineering of Cellular Environments: Cell Adhesion to Nano-Digital Surfaces
    PART II Subcellular Mechanical Properties and Activities
    6. Probing Cellular Mechanical Responses to Stimuli Using Ballistic Intracellular Nanorheology
    7. Multiple-Particle Tracking and Two-Point Microrheology in Cells
    8. Imaging Stress Propagation in the Cytoplasm of a Living Cell
    9. Probing Intracellular Force Distributions by High-Resolution Live Cell Imaging and Inverse Dynamics
    10. Analysis of Microtubule Curvature
    11. Nuclear Mechanics and Methods
    PART III Cellular and Embryonic Mechanical Properties and Activities
    12. The Use of Gelatin Substratcs for Traction Force Microscopy in Rapidly Moving Cells
    13. Microfabricated Silicone Elastomeric Post Arrays for Measuring Traction Forces of Adherent Cells
    14. Cell Adhesion Strengthening: Measurement and Analysis
    15. Studying the Mechanics of Cellular Processes by Atomic Force Microscopy
    16. Using force to probe single-molecule receptor-Cytoskeletal anchoring beneath the surface of a living cell
    17. High-Throughput Rheological Measurements with an Optical Stretcher
    18. Measuring Mechanical Properties of Embryos and Embryonic Tissues
    PART IV Mechanical Stimuli to Cells
    19. Tools to Study Cell Mechanics and Mechanotransduction
    20. Magnetic Tweezers in Cell Biology
    21. Optical Neuronal Guidance
    22. Microtissue Elasticity: Measurements by Atomic Force Microscopy and Its Influence on Cell Differentiation
    23. Demystifying the Effects of a Three-Dimensional Microenvironment in Tissue Morphogenesis