Methods in cell biology. v. 85, Fluorescent proteins / edited by Kevin F. Sullivan. — 2nd ed. — Amsterdam : Elsevier, c2008. – (58.1574/M592/v.85) |
Contents
CONTENTS
Preface
1. Autofluorescent Proteins
2. Functional Fusion Proteins by Random Transposon-Based GFP Insertion
3. Fluorescent Proteins for Photoactivation Experiments
4. Design and Optimization of Genetically Encoded Fluorescent Biosensors GTPase Biosensors
5. Fast 4D Microscopy
6. Single-Molecule Imaging of Fluorescent Proteins
7. Counting Kinetochore Protein Numbers in Budding Yeast Using Genetically Encoded Fluorescent Proteins
8. Fluorescent Protein Applications in Plants
9. Expression and Imaging of Fluorescent Proteins in the C. elegans Gonad and Early Embryo
10. Fluorescent Proteins in Zebrafish Cell and Developmental Biology
11. Identifying and Quantitating Neural Stem and Progenitor Cells in the Adult Brain
12. Using Fluorescent Proteins to Study mRNA Trafficking in Living Cells
13. Visualizing mRNA Localization and Local Protein Translation in Neurons
14. Quantitative FRAP in Analysis of Molecular Binding Dynamics In Vivo
15. Quantitative and Qualitative Analysis of Plant Membrane Traffic Using
Fluorescent Proteins
16. Engineering FRET Constructs Using CFP and YFP
17. Fluorescence Anisotropy Imaging Microscopy for Homo-FRET in Living Cells
18. FRET by Fluorescence Polarization Microscopy
19. Bimolecular Fluorescence Complementation: Visualization of Molecular Interactions in Living Cells
20. Protein-Protein Interactions Determined by Fluorescence Correlation Spectroscopy
21. Recent Advances on In Vivo Imaging with Fluorescent Proteins
22. Computational Processing and Analysis of Dynamic Fluorescence Image Data
23. Automated Classification of Mitotic Phenotypes of Human Cells Using Fluorescent Proteins
24. Open Tools for Storage and Management of Quantitative Image Data