Exocytosis and endocytosis / Andrei I. Ivanov. — Totowa, NJ : Humana Press, c2008. – (58.17/M592/v.440)

Contents

    Contents
    
    Preface
    Contributors
    Part I An Overview of Methods for Analyzing Vesicular Trafficking
    1 Vesicular Trafficking: Molecular Tools and Targets
    2 Pharmacological Inhibition of Endocytic Pathways: Is It Specific Enough to Be Useful?
    Part II Cell-Free and Biochemical Assays of Exocytosis and Endocytosis
    3 In Vitro Assays to Measure SNARE-Mediated Vesicle Fusion
    4 Imaging Ca2+-Triggered Exocytosis of Single Secretory Granules on Plasma Membrane Lawns from Neuroendocrine Cells
    5 FRAP Analysis of Secretory Granule Lipids and Proteins in the Sea Urchin Egg
    6 A Cell-Free Assay for Endocytosis of E-Cadherin
    7 Cell-Surface Biotinylation to Study Endocytosis and Recycling of Occludin
    8 Fractionation of Subcellular Membrane Vesicles of Epithelial and Nonepithelial Cells by OptiPrep Density Gradient Ultracentrifugation
    9 Identification and Characterization of Interacting Partners of Rab GTPases by Yeast Two-Hybrid Analyses
    Part III Analysis of Endocytosis and Exocytosis in Different Cell Systems
    10 High-Throughput Analysis of the Dynamics of Recycling Cell Surface Proteins
    11 Studying Phagocytosis by Live-Cell Scintillation Proximity Assay 147
    12 Transcytosis of Polymeric Immunoglobulin A in Polarized Madin-Darby Canine Kidney Cells 157
    13 The Use of Syntaxin Chimeras to Study Polarized Protein Trafficking in Epithelial Cells
    14 Targeting the Epithelial SNARE Machinery by Bacterial Neurotoxins 187
    15 Exocytosis of Endothelial Cells Is Regulated by N-Ethylmaleimide-Sensitive Factor 203
    16 Molecular Dissection of HCI Secretion in Gastric Parietal Cells Using Streptolysin O Permeabilization 217
    17 Addressing Membrane Protein Topology Using the Fluorescence Protease Protection (FPP) Assay 227
    18 Visualizing Clathrin-Mediated IgE Receptor Internalization by Electron and Atomic Force Microscopy 235
    19 Analyzing Endosomes in Nonsectioned Cells by Transmission Electron Microscopy
    20 Imaging Exocytosis of Single Insulin Secretory Granules With TIRF Microscopy
    21 Reconstitution of Depolarization and Ca2~-Evoked Secretion in Xenopus Oocytes Monitored by Membrane Capacitance 269
    22 Low-Noise Recording of Single-Vesicle Capacitance Steps in Cell-Attached Patches 283
    23 Good Practices in Single-Cell Amperometry 297
    24 Analysis of Single-Vesicle Exocytotic Events Recorded by Amperometry 315
    Part IV Analysis of Exocytosis and Endocytosis in Model Organisms
    25 Application of RNAi Technology and Fluorescent Protein Markers to Study Membrane Traffic in Caenorhabditis elegans 331
    26 FM 1-43 Labeling of Synaptic Vesicle Pools at the Drosophila Neuromuscular Junction 349
    27 Probing E-Cadherin Endocytosis by Morpholino-Mediated Rab5 Knockdown in Zebrafish 371
    28 Quantifying Endocytosis In Vivo Using Intravital Two-Photon Microscopy 389