Chromosomal mutagenesis / edited by Gregory D. Davis, Kevin J. Kayser. — Totowa, NJ : Humana Press, c2008. – (58.17/M592/v.435) |
Contents
Contents
Preface vii
Contributors xv
1 Biallelic Gene Knockouts in Chinese Hamster Ovary Cells
2 Highly Proficient Gene Targeting by Homologous Recombination in the Human Pre-B Cell Line Nalm-6
3 Robust Cell Line Development Using Meganucleases
4 Design and Testing of Zinc Finger Nucleases for Use in Mammalian Cells
5 Gene Targeting in Drosophila and Caenorhabditis elegans With Zinc-Finger Nucleases
6 Orpheus Recombination: A Comprehensive Bacteriophage System for Murine Targeting Vector Construction by Transplacement
7 Transposon-Mediated Mutagenesis in Somatic Cells: Identification of Transposon-Genomic DNA Junctions
8 Insertional Mutagenesis of the Mouse Germline With Sleeping Beauty Transposition
9 Conditional Gene Trapping Using the FLEx System
10 Steps Toward Targeted Insertional Mutagenesis With Class II Transposable Elements
11 Targeting Integration of the Saccharomyces Ty5 Retrotransposon
12 Site-Specific Chromosomal Integration Mediated by ΦC31 Integrase
13 Triplex-Mediated Gene Modification
14 Allelic Exchange of Unmarked Mutations in Mycobacterium Tuberculosis
15 Mycobacterial Recombineering
16 Chromosomal Engineering of Clostridium Perfringens Using Group II Introns