Drosophila : methods and protocols / edited by Christian Dahmann. — Totowa, N.J. : Humana Press, c2008. – (58.17/M592/v.420)

Contents

    Contents
    
    Preface v
    Contributors ix
    1 Drosophila melanogaster and the Development of Biology in the 20th Century
    2 Getting Started: An Overview on Raising and Handling Drosophila
    3 FlyBase: A Database for the Drosophila Research Community
    4 The Use of P-Element Transposons to Generate Transgenic Flies
    5 The GAL4 System: A Versatile System for the Expression of Genes
    6 P-Element Mutagenesis
    7 EMS Screens: From Mutagenesis to Screening and Mapping
    8 RNAi Screening in Cultured Drosophila Cells
    9 Methods for Homologous Recombination in Drosophila
    10 Recombinases and Their Use in Gene Activation， Gene Inactivation， and Transgenesis
    11 Cuticle Preparation of Drosophila Embryos and Larvae
    12 Immunolabeling of Embryos
    13 Imaging Cellular and Molecular Dynamics in Live Embryos Using Fluorescent Proteins
    14 Analysis of Neuromuscular Junctions: Histology and In Vivo Imaging
    15 Immunolabeling of Imaginal Discs
    16 Imaging Drosophila Pupal Wing Morphogenesis
    17 Microscopic Analysis of the Adult Drosophila Retina Using Semithin Plastic Sections
    18 Fluorescent In Situ Hybridization Protocols in Drosophila Embryos and Tissues
    19 Linear RNA Amplification for the Production of Microarray Hybridization Probes
    20 An Overview of the Identification， Detection， and Functional Analysis of Drosophila MicroRNAs
    21 Extraction and Immunoblotting of Proteins From Embryos
    22 Purification of Drosophila Protein Complexes for Mass Spectrometry
    23 Mass Production of Drosophila Embryos and Chromatographic Purification of Native Protein Complexes
    24 Flow Cytometric Analysis of Drosophila Cells
    25 Drosophila Cell Lines as Model Systems and as an Experimental Tool
    Appendix: Phenotypic Markers in Drosophila
    Index 429