RNAi : design and application / edited by Sailen Barik. — Totowa, N.J. : Humana Press, c2008. – (58.17/M592/v.442)

Contents

    Contents
    
    Preface vii
    Contributors xi
    PART I: DESIGNING OPTIMAL RNAI TOOLS
    1. Principles of Dicer Substrate (D-siRNA) Design and Function
    2. Expression， Purification， and Analysis of Recombinant Drosophila Dicer-1 and Dicer-2 Enzymes
    3. In vitro RNA Cleavage Assay for Argonaute-Family Proteins
    4. Identifying siRNA-Induced Off-Targets by Microarray Analysis
    PART I1: APPLICATION OF RNAI IN DIVERSE ORGANISMS
    5. Hydrodynamic Delivery of siRNA in a Mouse Model of Sepsis
    6. Nasal Delivery of siRNA
    7. RNA Interference as a Genetic Tool in Trypanosomes
    8. Lentivirus-Mediated RNA Interference in Mammalian Neurons
    9. Silencing Genes by RNA Interference in the Protozoan Parasite Entamoeba histolytica
    10. Use of RNAi in C. elegans
    11. Application of siRNA Against SARS in the Rhesus Macaque Model
    12. siRNA and shRNA as Anticancer Agents in a Cervical Cancer Model
    13. Identification and Expression Analysis of Small RNAs During Development
    14. Screening and Identification of Virus-Encoded RNA Silencing Suppressors
    15. Application of RNA Interference in Functional Genomics Studies of a Social Insect
    16. Temporal Control of Gene Silencing by in ovo Electroporation
    17. Altering Flower Color in Transgenic Plants by RNAi-Mediated Engineering of Flavonoid Biosynthetic Pathway
    18. Transgenic RNA Interference in Mice
    Index 267