Methods in cell biology. v. 84, Biophysical tools for biologists. v. 1, In vitro techniques / edited by John J. Correia, H. William Detrich, III. — Amsterdam : Elsevier, c2008. – (58.1574/M592/v.84)

Contents

    CONTENTS
    
    SECTION 1 Solution Methods
    1. Binding: A Polemic and Rough Guide
    2. Linked Equilibria in Regulation of Transcription Initiation
    3. Biosensor-Surface Plasmon Resonance Methods for Quantitative Analysis of Biomolecular Interactions
    4. Isothermal Titration Calorimetry: Experimental Design， Data Analysis， and Probing Macromolecule/Ligand Binding and Kinetic Interactions
    5. Differential Scanning Calorimetry
    6. Analytical Ultracentrifugation: Sedimcntation Velocity and Sedimentation Equilibrium
    7. Dctermmation of Membrane Protein Molecular Weights and Association Equilibrium Constants Using Sedimentation Equilibrium and Sedimentation Velocity
    8. Basic Aspects of Absorption and Fluorescence Spectroscopy and Resonance Energy Transfer Methods
    9. Applications of Fluorescence Anisotropy to the Study of Protein-DNA Interactions
    10. Circular 1)ichroism and Its Application to the Study of'Biomolecules
    11. Protein Folding and Stability Using Denaturants
    12. Hydrodynamic Modeling: The Solution Conformation of Macromoleculcs and Their Complexes
    13. X-Ray and Neutron Scattering Data and Their Constrained Molecular Modeling
    14. Structural Investigations into Microtubule-MAP Complexes
    15. Rapid Kinetic Techniqucs
    16. Mutagenic analysis of membrane protein functional mechanisms: bacteriorhodopsin as a model example
    17. Quantifying DNA-Proteiu Interactions by Single Molecule Stretching
    18. Isotopomer-Bascd Metabolomic Analysis by NMR and Mass SpectrometW
    19. Following Molecular Transitions with Single Residue Spatial and Millisecond Time Resolution
    20. Methods and Applications of Site-Directed Spin Labeling EPR Spectroscopy
    21. Eluorescence Correlation Spectroscopy and Its Application to the Characterization of Molecular Properties and Interactions
    22. A Practical Guide oi1 How Osmolytes Modulate Macromolecular Properties
    SECTION 2 Computational Methods
    23. Stupid Statistics
    24. Nonlinear Least-Squares Fitting Methods
    25. Methods for Sinmlating the Dynamics of Complex Biological Processes
    26. Computational Methods for Biomolecular Electrostatics
    27. Ligand Effects o21 the Protein Ensemble: Unifying the Descriptions of Ligand Binding， Local Conformational Fluctuations， and Protein Stability
    28. Molecular Modeling of the Cytoskeleton
    29. Mathematical Modeling of Cell Migration
    Index
    Volumes in Series