Post-transcriptional gene regulation / edited by Jeffrey Wilusz. — Totowa, N.J. : Humana Press, c2008. – (58.17/M592/v.419)

Contents

    Contents
    
    Preface v
    Contributors xv
    PART I BIOINFORMATICS
    1. Bioinformatics Approaches for Studying Untranslated Regions of mRNAs
    2. Identification of mRNA Polyadenylation Sites in Genomes Using cDNA Sequences， Expressed Sequence Tags， and Trace
    3. Bioinformatic Tools for Studying Post-Transcriptional Gene Regulation: The UAlbany TUTR Collection and Other Informatic Resources
    PART II: FUNDAMENTAL ASPECTS OF THE STUDY OF RNA BIOLOGY
    4. In-Line Probing Analysis of Riboswitches
    5. Ribotrap: Targeted Purification of RNA-Specific RNPs from Cell Lysates Through Immunoaffinity Precipitation to Identify Regulatory Proteins and RNAs
    6. Advances in RIP-Chip Analysis: RNA-Binding Protein Immunoprecipitation-Microarray Profiling
    7. Biosensors for RNA Aptamers--Protein Interaction
    8. A Tethering Approach to Study Proteins that Activate mRNA Turnover in Human Cells
    9. RNA Analysis by Biosynthetic Tagging Using 4-Thiouracil and Uracil Phosphoribosyltransferase
    10. Efficient 5’ Cap-Dependent RNA Purification: Use in Identifying and Studying Subsets of RNA
    PART III TECHNIQUES FOR SPECIFIC ASPECTS OF RNA BIOLOGY
    11. Enrichment of Alternatively Spliced Isoforms
    12. In Vivo Methods to Assess Polyadenylation Efficiency
    13. Monitoring the Temporal and Spatial Distribution of RNA in Living Yeast Cells
    14. Analysis of mRNA Partitioning Between the Cytosol and Endoplasmic Reticulum Compartments of Mammalian Cells
    15. In Vivo and In Vitro Analysis of Poly(A) Length Effects on mRNA Translation
    16. A Ribosomal Density-Mapping Procedure to Explore Ribosome Positions Along Translating mRNAs
    17. Identification of Changes in Gene Expression by Quantitation of mRNA Levels
    18 Application of the Invader~ RNA Assay to the Polarity of Vertebrate mRNA Decay
    19. Development of an In Vitro mRNA Decay System in Insect Cells
    20. Using Synthetic Precursor and Inhibitor miRNAs to Understand miRNA Function
    21. A Step-by-Step Procedure to Analyze the Efficacy of siRNA Using Real-Time PCR