Gene transfer : delivery and expression of DNA and RNA : a laboratory manual / edited by Theodore Friedmann, John Rossi. — Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2007. – (58.21/G326n) |
Contents
Contents
1 Introduction, 1
VIRAL VECTORS
2 Retroviral Vectors, 3
3 Development of Lentiviral Vectors Expressing siRNA, 23
4 HIV-2 Vectors in Human Gene Therapy: Design, Construction, and Therapuetic Potential, 35
5 SIV Vectors as Vehicles for DNA Delivery, 47
6 Production and Use of Feline Immunodeficiency Virus-based Lentiviral Vectors, 57
7 Lentivirus Transduction of Hematopoietic Cells, 75
8 Spleen Necrosis Virus-based Vectors, 83
9 Foamy Virus Vector Production and Transduction of Hematopoietic Cells, 91
10 Simian Foamy Virus Type-1 Vectors, 99
11 Generation of VSV-G-pseudotyped Retroviral Vectors, 107
12 Targeted Gene Transfer with Surface-engineered Lentiviral Vectors, 119
13 Preparation of Pseudotyped Lentiviral Vectors Resistant to Inactivation by Serum Complement, 131
14 Generation of 2A Peptide-linked Multicistronic Vectors, 137
15 Construction of First-generation Adenoviral Vectors, 149
16 Production and Characterization of Helper-dependent Adenoviral Vectors, 167
17 Cell and Tissue Targeting, 183
18 Stable Producer Cell Lines for AAV Assembly, 197
19 Strategies for the Design of Hybrid Adeno-associated Virus Vectors, 203
20 Recombinant Herpes Simplex Virus Vectors, 217
21 Herpes Simplex Virus Type-1-derived Amplicon Vectors, 227
22 7-2 Herpesvirus Saimiri-based Vectors, 255
23 Gene Delivery Using HSV/AAV Hybrid Amplicon Vectors, 265
24 Polyomaviruses: SV40, 273
25 SV40 In Vitro Packaging: A Pseudovirion Gene Delivery System, 289
26 Baculovirus-based Display and Gene Delivery Systems, 297
27 Safe, Simple, and High-capacity Gene Delivery into Insect and Vertebrate Cells by Recombinant Baculoviruses, 313
28 Alphaviruses: Semliki Forest Virus and Sindbis Virus as Gene Delivery Vectors, 327
29 Gene Transfer into Mammalian Cells Using Targeted Filamentous Bacteriophage, 349
30 Selection, Isolation, and Identification of Targeting Peptides for Ligand-directed Gene Delivery, 359
31 Rescue and Propagation of Tropism-modified Measles Viruses, 371
32 Picornavirus-based Expression Vectors, 381
33 Reverse Genetics of Influenza Viruses, 389
34 An Overview of Condensing and Noncondensing Polymeric Systems for Gene Delivery, 395
35 Transfection of Hippocampal Neurons with Plasmid DNA Using Calcium Phosphate Coprecipitation, 405
36 Gene Delivery to Skin Using Biolistics, 411
37 Optimizing Electrotransfection of Mammalian Cells In Vitro, 419
38 Micro In Utero Electroporation for Efficient Gene Targeting in Mouse Embryos, 427
39 Lipoplex and LPD Nanoparticles for In Vivo Gene Delivery, 433
40 Bioresponsive Targeted Charge Neutral Lipid Vesicles for Systemic Gene Delivery, 441
41 HVJ Liposomes and HVJ Envelope Vectors, 451
42 Polylysine Copolymers for Gene Delivery, 461
43 PEI Nanoparticles for Targeted Gene Delivery, 473
44 Cyclodextrin-containing Polycations for Nucleic Acid Delivery, 479
45 Bionanocapsules Using the Hepatitis B Virus Envelope L Protein, 487
46 Formulations of Solid Lipid Nanoparticles for Transfection of Mammalian Cells In Vitro, 491
47 PEGylated Poly-L-lysine DNA Nanoparticles, 497
48 Water-soluble Lipopolymers and Lipopeptides for Nucleic Acid Delivery, 501
49 Cationic Polysaccharides for DNA Delivery, 507
50 Sustained Release of Plasmid DNAs Encoding Platelet-derived Growth Factor and Hyaluronan Synthase 2 from Cross-linked Hyaluronan Matrices and Films, 515
51 Linear Polyethylenimine: Synthesis and Transfection Procedures for In Vitro and In Vivo, 521
52 Protein Nanospheres for Gene Delivery: Preparation and In Vitro Transfection Studies with Gelatin Nanoparticles, 527
53 Vesicular Stomatitis Virus-G Conjugate, 541
54 High-throughput Methods for Screening Polymeric Transfection Reagents, 547
55 Poly(Lactic Acid) and Poly(Ethylene Oxide) Nanoparticles as Carriers for Gene Delivery, 555
56 Biodegradable Nanoparticles, 561
57 Transposon-mediated Delivery of Small Interfering RNA: Sleeping Beauty Transposon, 567
58 Efficient DNA Delivery into Mammalian Cells by Displaying the TAT Transduction Domain on Bacteriophage λ, 573
59 Cell-penetrating Peptide-mediated Delivery of Peptide Nucleic Acid Oligomers, 581
60 Conditional Mutagenesis of the Genome Using Site-specific DNA Recombination, 587
61 Expression and Validation of Ribozyme and Short Hairpin RNA in Mammalian Cells, 603
62 Mifepristone-inducible Gene Regulatory System, 615
63 Dimerizer-mediated Regulation of Gene Expression, 631
65 Site-specific Integration with Phage ФC31 Integrase, 653
66 Creating Zinc Finger Nucleases to Manipulate the Genome in a Site-specific Manner Using a Modular-assembly Approach, 661
67 Assembly of De Novo Bacterial Artificial Chromosome-based Human Artificia Chromosomes, 675
68 Delivery of Naked DNA Using Hydrodynamic Injection Techniques, 691
69 Nonviral Gene Transfer across the Blood-brain B.arrier with Trojan Horse Liposomes, 701
70 Sonoporation: An Efficient Technique for the Introduction of Genes into Chick Embryos, 711
71 Genetic Manipulation of Mammalian Cells by Microinjection, 717
72 Magnetofection, 723
73 Photochemical Internalization for Light-directed Gene Delivery, 731
74 Pronuclear Microinjection in Mice, 739
75 Knockdown Transgenic Mice Generated by Silencing Lentiviral Vectors, 755