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Gene transfer : delivery and expression of DNA and RNA : a laboratory manual / edited by Theodore Friedmann, John Rossi. — Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2007. – (58.21/G326n)

Contents

        Contents
    
    1 Introduction, 1
    VIRAL VECTORS
    2 Retroviral Vectors, 3
    3 Development of Lentiviral Vectors Expressing siRNA, 23
    4 HIV-2 Vectors in Human Gene Therapy: Design, Construction, and Therapuetic Potential, 35
    5 SIV Vectors as Vehicles for DNA Delivery, 47
    6 Production and Use of Feline Immunodeficiency Virus-based Lentiviral Vectors, 57
    7 Lentivirus Transduction of Hematopoietic Cells, 75
    8 Spleen Necrosis Virus-based Vectors, 83
    9 Foamy Virus Vector Production and Transduction of Hematopoietic Cells, 91
    10 Simian Foamy Virus Type-1 Vectors, 99
    11 Generation of VSV-G-pseudotyped Retroviral Vectors, 107
    12 Targeted Gene Transfer with Surface-engineered Lentiviral Vectors, 119
    13 Preparation of Pseudotyped Lentiviral Vectors Resistant to Inactivation by Serum Complement, 131
    14 Generation of 2A Peptide-linked Multicistronic Vectors, 137
    15 Construction of First-generation Adenoviral Vectors, 149
    16 Production and Characterization of Helper-dependent Adenoviral Vectors, 167
    17 Cell and Tissue Targeting, 183
    18 Stable Producer Cell Lines for AAV Assembly, 197
    19 Strategies for the Design of Hybrid Adeno-associated Virus Vectors, 203
    20 Recombinant Herpes Simplex Virus Vectors, 217
    21 Herpes Simplex Virus Type-1-derived Amplicon Vectors, 227
    22 7-2 Herpesvirus Saimiri-based Vectors, 255
    23 Gene Delivery Using HSV/AAV Hybrid Amplicon Vectors, 265
    24 Polyomaviruses: SV40, 273
    25 SV40 In Vitro Packaging: A Pseudovirion Gene Delivery System, 289
    26 Baculovirus-based Display and Gene Delivery Systems, 297
    27 Safe, Simple, and High-capacity Gene Delivery into Insect and Vertebrate Cells by Recombinant Baculoviruses, 313
    28 Alphaviruses: Semliki Forest Virus and Sindbis Virus as Gene Delivery Vectors, 327
    29 Gene Transfer into Mammalian Cells Using Targeted Filamentous Bacteriophage, 349
    30 Selection, Isolation, and Identification of Targeting Peptides for Ligand-directed Gene Delivery, 359
    31 Rescue and Propagation of Tropism-modified Measles Viruses, 371
    32 Picornavirus-based Expression Vectors, 381
    33 Reverse Genetics of Influenza Viruses, 389
    34 An Overview of Condensing and Noncondensing Polymeric Systems for Gene Delivery, 395
    35 Transfection of Hippocampal Neurons with Plasmid DNA Using Calcium Phosphate Coprecipitation, 405
    36 Gene Delivery to Skin Using Biolistics, 411
    37 Optimizing Electrotransfection of Mammalian Cells In Vitro, 419
    38 Micro In Utero Electroporation for Efficient Gene Targeting in Mouse Embryos, 427
    39 Lipoplex and LPD Nanoparticles for In Vivo Gene Delivery, 433
    40 Bioresponsive Targeted Charge Neutral Lipid Vesicles for Systemic Gene Delivery, 441
    41 HVJ Liposomes and HVJ Envelope Vectors, 451
    42 Polylysine Copolymers for Gene Delivery, 461
    43 PEI Nanoparticles for Targeted Gene Delivery, 473
    44 Cyclodextrin-containing Polycations for Nucleic Acid Delivery, 479
    45 Bionanocapsules Using the Hepatitis B Virus Envelope L Protein, 487
    46 Formulations of Solid Lipid Nanoparticles for Transfection of Mammalian Cells In Vitro, 491
    47 PEGylated Poly-L-lysine DNA Nanoparticles, 497
    48 Water-soluble Lipopolymers and Lipopeptides for Nucleic Acid Delivery, 501
    49 Cationic Polysaccharides for DNA Delivery, 507
    50 Sustained Release of Plasmid DNAs Encoding Platelet-derived Growth Factor and Hyaluronan Synthase 2 from Cross-linked Hyaluronan Matrices and Films, 515
    51 Linear Polyethylenimine: Synthesis and Transfection Procedures for In Vitro and In Vivo, 521
    52 Protein Nanospheres for Gene Delivery: Preparation and In Vitro Transfection Studies with Gelatin Nanoparticles, 527
    53 Vesicular Stomatitis Virus-G Conjugate, 541
    54 High-throughput Methods for Screening Polymeric Transfection Reagents, 547
    55 Poly(Lactic Acid) and Poly(Ethylene Oxide) Nanoparticles as Carriers for Gene Delivery, 555
    56 Biodegradable Nanoparticles, 561
    57 Transposon-mediated Delivery of Small Interfering RNA: Sleeping Beauty Transposon, 567
    58 Efficient DNA Delivery into Mammalian Cells by Displaying the TAT Transduction Domain on Bacteriophage λ, 573
    59 Cell-penetrating Peptide-mediated Delivery of Peptide Nucleic Acid Oligomers, 581
    60 Conditional Mutagenesis of the Genome Using Site-specific DNA Recombination, 587
    61 Expression and Validation of Ribozyme and Short Hairpin RNA in Mammalian Cells, 603
    62 Mifepristone-inducible Gene Regulatory System, 615
    63 Dimerizer-mediated Regulation of Gene Expression, 631
    65 Site-specific Integration with Phage ФC31 Integrase, 653
    66 Creating Zinc Finger Nucleases to Manipulate the Genome in a Site-specific Manner Using a Modular-assembly Approach, 661
    67 Assembly of De Novo Bacterial Artificial Chromosome-based Human Artificia Chromosomes, 675
    68 Delivery of Naked DNA Using Hydrodynamic Injection Techniques, 691
    69 Nonviral Gene Transfer across the Blood-brain B.arrier with Trojan Horse Liposomes, 701
    70 Sonoporation: An Efficient Technique for the Introduction of Genes into Chick Embryos, 711
    71 Genetic Manipulation of Mammalian Cells by Microinjection, 717
    72 Magnetofection, 723
    73 Photochemical Internalization for Light-directed Gene Delivery, 731
    74 Pronuclear Microinjection in Mice, 739
    75 Knockdown Transgenic Mice Generated by Silencing Lentiviral Vectors, 755