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Single-molecule techniques : a laboratory manual / edited by Paul R. Selvin, Taekjip Ha. — Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2008. – (58.178073/S617) |
Contents
Contents
Preface， vii
1 The New Era of Biology In Singulo， 1
IN VITRO FLUORESCENCE
2 Single-Molecule FRET with Total Internal Reflection Microscopy， 3
3 In Vitro and In Vivo FIONA and Other Acronyms for Watching Molecular Motors Walk， 37
4 Colocalization of Fluorescent Probes: Accurate and Precise Registration with Nanometer Resolution， 73
5 Alternating-Laser Excitation of Single Molecules， 85
6 Orientation and Rotational Motions of Single Molecules by Polarized Tota Internal Reflection Fluorescence Microscopy， 121
FLUORESCENCE IN LIVING CELLS
7 Imaging Gene Expression in Living Cells at the Single-Molecule Level， 149
8 Single-Virus Tracking in Live Cells， 171
9 Ultrasensitive Imaging in Live Cells Using Fluorescent Quantum Dots， 193
10 Imaging Real-Time Gene Expression in Living Systems， 209
11 Single-Molecule Imaging of Stochastic Signaling Events in Living Cells， 239
12 Fluorescence Correlation Spectroscopy In Vitro and In Vivo， 259
OPTICAL TRAPS
13 Optical Traps to Study Properties of Molecular Motors， 279
14 High-Resolution Dual-Trap Optical Tweezers with Differential Detection， 297
15 Imaging and Nanomanipulation of an Actomyosin Motor， 325
MAGNETIC TRAPS
16 Single-Molecule Studies Using Magnetic Traps， 347
FORCE PROBES/ATOMIC FORCE MICROSCOPY
17 Probing Polysaccharide and Protein Mechanics by Atomic Force Microscopy， 371
CHANNELS
18 Single-Molecule Analysis of Nucleic Acids and DNA-Protein Interactions Using Nanopores， 395
OTHER TECHNIQUES
19 Single-Molecule Gold-Nanoparticle Tracking with High Temporal and Spatial Resolution and without an Applied Load， 421
20 Advances in Surface-based Assays for Single Molecules， 431
21 Hydrodynamic Flow-stretching Assay for Single-Molecule Studies of Nucleic Acid-Protein Interactions， 461
APPENDIX: Cautions， 493
Index， 501
