Immunology / Werner Luttmann ... [et al.]. — Amsterdam : Elsevier, c2006. – (58.691/L974) |
Contents
Contents
Foreword IX
Excursuses XI
Abbreviations XIII
1 Antibodies
1.1 Antibody Characteristics
1.2 Fabrication of Antibodies
1.3 Purification of Antibodies
1.4 Chemical Coupling and Marking of Antibodies 30
2 Cell Separation
2.1 Separation by Cell Size and Cell Density: Centrifugation Techniques
2.2 Separation by Means of Cell-Specific Surface Molecules
3 Flow Cytometry
3.1 How Does That Work?
3.2 Fluorescences
3.3 Sample Preparation
3.4 Start-Up of the Flow Cytometer
3.5 Compensation and Measuring
3.6 Analysis
3.7 Models and Configurations
4 Quantitative Immunoassays
4.1 Assay Concepts
4.2 Radioimmunoassay (RIA)
4.3 Enzyme-Linked Immunosorbent Assay (ELISA)
4.4 ELISPOT Assay 98
4.5 Particle Immunoassay (PIA)
4.6 Amplification Systems
5 Western Blot
5.1 Sample Preparation
5.2 Protein Separation by Gel Electrophoresis
5.3 Transfer of Proteins to a Membrane (Blot)
5.4 Protein Detection
5.5 Dot and Slot Blot
6 In situ lmmunolocalization
6.1 Analyses of Cell Suspensions
6.2 Tissue Analysis
6.3 Immunoelectron Microscopic Tissue Analysis
7 lmmunoprecipitation 153
7.1 The Classics 154
7.2 Immunoprecipitation "Today"
8 The Cell: Live, Eat, Die
8.1 Cell Viability
8.2 Cell Proliferation
8.3 Phagocytosis Assays
8.4 Cell-Mediated Cytotoxicity
8.5 Apoptosis Assays 180
9 Special Immunoassays 189
9.1 Determination of Blood Groups 189
9.2 HLA Typing 192
9.3 Lymphoblast Transformation 195
10 A Short Excursion into the Unbeloved World of Statistics 197
10.1 Descriptive Statistics
10.2 Inferential Statistics
Appendix: CD-Antigens
Glossary 225
Index