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新书资源(2008年8月)

Immunology / Werner Luttmann ... [et al.]. — Amsterdam : Elsevier, c2006. – (58.691/L974)

Contents

    Contents
    
    Foreword IX
    Excursuses XI
    Abbreviations XIII
    1 Antibodies
    1.1 Antibody Characteristics
    1.2 Fabrication of Antibodies
    1.3 Purification of Antibodies
    1.4 Chemical Coupling and Marking of Antibodies 30
    2 Cell Separation
    2.1 Separation by Cell Size and Cell Density: Centrifugation Techniques
    2.2 Separation by Means of Cell-Specific Surface Molecules
    3 Flow Cytometry
    3.1 How Does That Work?
    3.2 Fluorescences
    3.3 Sample Preparation
    3.4 Start-Up of the Flow Cytometer
    3.5 Compensation and Measuring
    3.6 Analysis
    3.7 Models and Configurations
    4 Quantitative Immunoassays
    4.1 Assay Concepts
    4.2 Radioimmunoassay (RIA)
    4.3 Enzyme-Linked Immunosorbent Assay (ELISA)
    4.4 ELISPOT Assay 98
    4.5 Particle Immunoassay (PIA)
    4.6 Amplification Systems
    5 Western Blot
    5.1 Sample Preparation
    5.2 Protein Separation by Gel Electrophoresis
    5.3 Transfer of Proteins to a Membrane (Blot)
    5.4 Protein Detection
    5.5 Dot and Slot Blot
    6 In situ lmmunolocalization
    6.1 Analyses of Cell Suspensions
    6.2 Tissue Analysis
    6.3 Immunoelectron Microscopic Tissue Analysis
    7 lmmunoprecipitation 153
    7.1 The Classics 154
    7.2 Immunoprecipitation "Today"
    8 The Cell: Live, Eat, Die
    8.1 Cell Viability
    8.2 Cell Proliferation
    8.3 Phagocytosis Assays
    8.4 Cell-Mediated Cytotoxicity
    8.5 Apoptosis Assays 180
    9 Special Immunoassays 189
    9.1 Determination of Blood Groups 189
    9.2 HLA Typing 192
    9.3 Lymphoblast Transformation 195
    10 A Short Excursion into the Unbeloved World of Statistics 197
    10.1 Descriptive Statistics
    10.2 Inferential Statistics
    Appendix: CD-Antigens
    Glossary 225
    Index