Avidin-biotin interactions : methods and applications / edited by Robert J. McMahon. — Totowa, NJ : Humana, c2008. – (58.17/M592/v.418) |
Contents
Contents
Preface
Contributors
1. Preparation of Avidin Conjugates
2. Coupling of Antibodies With Biotin
3. Optimization of Detection and Quantification of Proteins on Membranes in Very High and Very Low Abundance Using Avidin and Streptavidin
4. Use of the Avidin (Imino) Biotin System as a General Approach to Affinity Precipitation
5. Capturing Biotinylated Proteins and Peptides by Avidin Functional Affinity Electrophoresis
6. Functionality Screen of Streptavidin Mutants by Non-Denaturing SDS-PAGE Using Biotin-4-Fluorescein
7. Application of Biotin-4-Fluorescein in Homogeneous Fluorescence Assays for Avidin, Streptavidin, and Biotin or Biotin Derivatives
8. Quantitative Recovery of Biotinylated Proteins from Streptavidin-Based Affinity Chromatography Resins
9. High-Yield Production and Purification of Recombinant T7-Tag Mature Streptavidin in Glucose-Stressed E. coli
10. Endogenous Biotin in Rat Brain: Implications for False-Positive Results With Avidin-Biotin and Streptavidin-Biotin Techniques
11. Pseudo-lmmunolabelling With the Avidin-Biotin-Peroxidase Complex due to the Presence of Endogenous Biotin in the Retina
12. Use of Synthetic Peptides for Identifying Biotinylation Sites in Human Histones
13. Detection and Quantitation of the Activity of DNA Methyltransferases Using a Biotin/Avidin Microplate Assay
14. Anti-Biotin Antibodies Offer Superior Organelle-Specific Labelling of Mitochondria Over Avidin or Streptavidin
15. Mammalian Lectin as Tool in Glycochemistry and Histochemistry With Relevance for Diagnostic Procedure
16. A Multiplex Approach to Isotyping Antigen-Specific Antibodies Using Biotinylated Antigen/Streptavidin-Phycoerythrin
17. Biotin-Protein Bond: Instability and Structural Modification to Provide Stability for In Vivo Applications