Alcohol : methods and protocols / Laura E. Nagy, editor. — Totowa, NJ : Humana Press, c2008. – (58.17/M592/v.447)

Contents

    Contents
    
    Preface v
    Acknowledgments vii
    Contributors xiii
    Part I Animal Models of Acute and Chronic Ethanol Exposure
    1 Acute Models of Ethanol Exposure to Mice
    2 A Voluntary Oral-feeding Rat Model for Pathological Alcoholic Liver Injury 11
    3 Intragastric Ethanol Infusion Model in Rodents 33
    4 A Practical Method of Chronic Ethanol Administration in Mice 49
    Part II Animal Models of Ethanol Exposure During Development
    5 Analysis of Ethanol Developmental Toxicity in Zebrafish 63
    6 The Avian Embryo in Fetal Alcohol Research 75
    7 Artificial Rearing
    8 Intragastric Intubation of Alcohol During the Perinatal Period 101
    Part III Cell Culture Approaches to Studying Ethanol Exposure
    9 Human Monocytes， Macrophages， and Dendritic Cells: Alcohol Treatment Methods 113
    10 Generation and Use of Primary Rat Cultures for Studies of the Effects of Ethanol 125
    11 Development and Properties of HepG2 Cells That Constitutively Express CYP2E1
    12 Modeling the Impact of Alcohol on Cortical Development in a Dish: Strategies from Mapping Neural Stem Cell Fate 151
    Part IV Regulation of Specific Organ Systems in Response to Ethanol
    13 Acetaldehyde-induced Barrier Disruption and Paracellular Permeability in Caco-2 Cell Monolayer
    14 Alcohol-induced Oxidative Stress in the Liver: In Vivo Measurements
    15 Isolation of Kupffer Cells from Rats Fed Chronic Ethanol 199
    16 Dendritic Ceils in Chronic In Vivo Ethanol Exposure Models 213
    17 Formation and Immunological Properties of Aldehyde-derived Protein Adducts following Alcohol Consumption 235
    18 Assessment of Natural Killer (NK) and NKT Cells in Murine Spleens and Livers
    19 Polyclonal and Antigen-specific Responses of T Cells and T Cell Subsets 277
    20 B-Cell Studies in Chronic Ethanol Mice 295
    21 Histological Analysis of Bone 325
    22 Assessing Effects of Alcohol Consumption on Protein Synthesis in Striated Muscles 343
    23 Methods to Investigate the Effects of Chronic Ethanol on Adipocytes 357
    Part V Proteomic and Genomic Approaches to Study Response to Ethanol
    24 Proteomic Approaches to Identify and Characterize Alterations to the Mitochondrial Proteome in Alcohofic Liver Disease
    25 Alcoholic Liver Disease and the Mitochondrial Ribosome: Methods of Analysis
    26 Microarray Analysis of Ethanol-Induced Changes in Gene Expression 395