Glycobiology protocols / edited by Inka Brockhausen. — Totowa, N.J. : Humana Press, c2006. – (58.17/M592/v.347)

Contents

    Contents
    
    Preface
    Contributors
    1. Application of Fluorophore-Assisted Carbohydrate Electrophoresis for the Study of the Dolichol Pyrophosphate-Linked Oligosaccharides Pathway in Cell Cultures and Animal Tissues 1
    2. Partial Purification of Mannosylphosphorylundecaprenol Synthase From Micrococcus luteus: A Useful Enzyme for the Biosynthesis of a Variety of Mannosylphosphorylpolyisoprenol Products 13
    3. Assay of DolichyI-Phospho-Mannose Synthase Reconstituted in a Lipid Matrix 31
    4. O-Mannosylation in Mammalian Cells
    5. Methods for Analysis of Unusual Forms of O-Glycosylation 57
    6. Structural Analysis of Naturally Occurring Sialic Acids 69
    7. Nonradioactive Trans-Sialidase Screening Assay 93
    8. Structural Determination of O-Glycans by Tandem Mass Spectrometry 109
    9. Detailed Structural Analysis of N-Glycans Released From Glycoproteins in SDS-PAGE Gel Bands Using HPLC Combined With Exoglycosidase Array Digestions 125
    10. Molecular Modeling of Glycosyltransferases 145
    11. β-Galactoside α2，6-Sialyltransferase and the Sialyl α2，6-GalactosyI-Linkage in Tissues and Cell Lines 157
    12. Visualizing Intracellular Distribution and Activity of Core2 β(1，6)N-Acetylglucosaminyltransferase-I in Living Cells 171
    13. Gene Expression Analysis of Glycosylation-Related Genes by Real-Time Polymerase Chain Reaction 187
    14. Analysis of the Glycodynamics of Primary Osteoblasts and Bone Cancer Cells 211
    15. Micromethods for the Characterization of Lipid A-Core and O-Antigen Lipopolysaccharide 237
    16. Assay for a Galactosyltransferase Involved in the Assembly of the O7-Antigen Repeat Unit of Escherichia coli 253
    17. High-Th roughput Quantitation of Metabolically Labeled Anionic Glycoconjugates by Scintillation Proximity Assay Utilizing Binding to Cationic Dyes
    18. Quantitative Analysis of Mucins in Mucosal Secretions Using Indirect Enzyme-Linked Immunosorbent Assay
    19. Molecular Dissection of the Mouse Zona Pellucida: An Electron-Microscopic Perspective Utilizing High-Resolution Colloidal-Gold Labeling Methods
    20. Soluble Adamantyl Glycosphingolipid Analogs as Probes of Glycosphingolipid Function
    21. Diagnosis of Krabbe Disease by Use of a Natural Substrate
    22. In Vitro Assays of the Functions of Calnexin and Calreticulin， Lectin Chaperones of the Endoplasmic Reticulum
    23. Quantitative Measurement of Selectin-Ligand Interactions: Assays to Identify a Sweet Pill in a Library of Carbohydrates
    24. Binding and Inhibition Assays for Siglecs 359