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Mitochondria : Practical protocols / edited by Dario Leister, Johannes M. Herrmann. — Totowa, N.J. : Humana press, 2007. – (58.17/M592/v.372) |
Contents
Contents
Preface
Contributors
I MODEL SYSTEMS TO STUDY MITOCHONDRIAE FUNCTIONS
1 The Mitochondria of Cultured Mammalian Cells: I: Analysis by Immunofluorescence Microscopy， Histochemistry， Subcellular Fractionation， and Cell Fusion
2 The Mitochondria of Cultured Mammalian Cells: II: Expression and Visualization of Exogenous Proteins in Fixed and Live Cells
3 Drosophila melanogaster as a Model System to Study Mitochondrial Biology
4 Isolation and Functional Analysis of Mitochondria From the Nematode Caenorhabditis elegans
5 Isolation of Mitochondria From Procyclic Trypanosoma brucei
6 Saccharomyces cerevisiae as a Model Organism to Study Mitochondrial Biology: General Considerations and Basic Procedures
7 Studying Mitochondria in An Attractive Model: Schizosaccharomyces pombe
8 Neurospora crassa as a Model Organism for Mitochondrial Biogenesis
9 Isolation of Intact， Functional Mitochondria From the Model Plant Arabidopsis thaliana
10 Chlamydomonas reinhardtii: The Model of Choice to Study Mitochondria From Unicellular Photosynthetic Organisms
II GENETICS AND MOLECULAR BIOLOGY
11 Directed Alteration of Saccharomyces cerevisiae Mitochondrial DNA by Biolistic Transformation and Homologous Recombination
12 Screens for Mitochondrial Mutants in Yeast
13 Transcript End Mapping and Analysis of RNA Editing in Plant Mitochondria
14 In Vitro Analysis of the Yeast Mitochondrial RNA Polymerase
15 Functional Analysis by Inducible RNA Interference in Drosophila melanogaster
16 Analysis of Replicating Mitochondrial DNA by Two-Dimensional Agarose Gel Electrophoresis
III BIOCHEMISTRY
17 The Analysis of tRNA Import Into Mammalian Mitochondria
18 Analysis of Mitochondrial Protein Synthesis in Yeast
19 Large-Scale Isolation of Mitochondrial Ribosomes From Mammalian Tissues
20 Protein Import Into Isolated Mitochondria
21 Analyzing Import Intermediates of Mitochondrial Proteins by Blue Native Gel Electrophoresis
22 In Vitro and In Vivo Protein Import Into Plant Mitochondria
23 Two-Dimensional Blue Native/Blue Native Polyacrylamide Gel Electrophoresis for the Characterization of Mitochondrial Protein Complexes and Supercomplexes
24 Investigation of Iron-Sulfur Protein Maturation in Eukaryotes
25 Studying Proteolysis Within Mitochondria
IV PHYSIOLOGY
26 Methods to Determine the Status of Mitochondrial ATP Synthase Assembly
27 ATP Production in Isolated Mitochondria of Procyclic Trypanosoma brucei
28 Oxidative Stress and Plant Mitochondria
29 Measuring Mitochondrial Shape Changes and Their Consequences on Mitochondrial Involvement During Apoptosis
30 Assessing Mitochondrial Potential， Calcium， and Redox State in Isolated Mammalian Cells Using Confoca[ Microscopy
V DYNAMICS AND MORPHOLOGY
31 Fluorescence Imaging of Mitochondria in Yeast
32 Mitochondrial Fusion In Vitro
33 Conventional and Immunoelectron Microscopy of Mitochondria
34 Mitochondrial Division in Caenorhabditis elegans
Vl FUNCTIONAL GENOMICS
35 Yeast Mitochondrial Transcriptomics
36 Plant Mitochondrial Transcriptomics by Quantitative RT-PCR
37 Proteomics of Yeast Mitochondria
38 Arabidopsis Mitochondrial Proteomics
39 MitoP2， an Integrated Database for Mitochondrial Proteins
Index 587
