Methods for general and molecular microbiology / C.A. Reddy, editor in chief ; T.J. Beveridge ... [et al.], editors. — 3rd ed. — Washington, D.C. : ASM Press, c2007. – (58.671057/M592/3rd ed.)

Contents

    CONTENTS
    
    SECTION I MORPHOLOGY AND ULTRASTRUCTURE / 1
    Introduction to Morphology and Ultrastructure / 3
    1. Light Microscopy / 5
    2. Sampling and Staining for Light Microscopy / 19
    3. Laser Scanning Microscopy / 34
    4. Electron Microscopy / 54
    5. Computational Image Analysis and Reconstruction from Transmission Electron Micrographs / 82
    6. Atomic Force Microscopy / 96
    7. Cell Fractionation / 108
    8. Antigen-Antibody Reactions / 138
    SECTION II GROWTH/ 169
    Introduction / 171
    9. Growth Measurement / 172
    10. Nutrition and Media / 200
    11. Enrichment and Isolation / 215
    12. Culture Techniques / 270
    13. Energetics， Stoichiometry， and Kinetics of Microbial Growth / 286
    14. Physicochemical Factors in Growth / 309
    15. Phenotypic Characterization and the Principles of Comparative Systematics / 330
    16. General Methods To Investigate Microbial Symbioses / 394
    SECTION METABOLISM / 421
    Introduction to Metabolism / 423
    17. Physical Analysis and Purification Methods / 424
    18. Chemical Analysis / 462
    19. Enzymatic Activity / 504
    20. Permeability and Transport / 527
    21. Bacterial Respiration / 539
    22. Carbohydrate Fermentations / 558
    23. Metabolism of Aromatic Compounds / 586
    24. Cellulases， Hemicellulases， and Pectinases / 596
    25. Lignin and Lignin-Modifying Enzymes / 611
    SECTION IV MOLECULAR GENETICS / 621
    Introduction to Molecular Genetics / 623
    26. Similarity Analysis of DNAs / 624
    27. Nucleic Acid Analysis / 653
    28. Measuring Spontaneous Mutation Rates / 676
    29. Transposon Mutagenesis / 684
    30. Plasmids / 709
    31. GeneTransfer in Gram-Negative Bacteria / 735
    32. Genetic Exchange in Gram-Positive Bacteria / 756
    33. Genetics of Archaea / 800
    34. Genetic Manipulations Using Phages / 825
    SECTION V COMMUNITY AND GENOMIC ANALYSIS / 839
    Introduction to Community and Genomic Analysis / 841
    35. Characterization of Bacterial Genome Sequences by Similarity Searching / 842
    36. Reconstructing and Interpreting Evolutionary Relationships / 856
    37. Microbial Nucleotide Fingerprints in Nature / 869
    38. Construction of BAC and Fosmid Libraries from Naturally Occurring Microbial Populations / 879
    39. Single Cell Identification by Fluorescence In Situ Hybridization / 886
    40. Measurement of rRNA Abundance by Hybridization with Oligodeoxynucleotide Probes / 897
    41. Analysis of Microbial Communities with Denaturing Gradient Gel Electrophoresis and Terminal Restriction Fragment Length Polymorphism / 909
    SECTION V MYCOLOGY / 925
    Introduction to Mycology / 927
    42. Methods for Studying Terrestrial Fungal Ecology and Diversity / 929
    43. Physiology， Metabolism， and Molecular Aspects of Filamentous Fungi / 952
    44. Microbiolegical and Genetic Methods for Filamentous Fungi / 965
    45. Principles and Practice of DNA MicroarrayTechnology / 978
    APPENDICES
    46. Laboratory Safety / 997
    47. Culture Preservation / 1019
    Author Index / 1035
    Subject Index / 1037