Serial analysis of gene expression (SAGE) : methods and protocols / edited by Kare Lehmann Nielsen. — Totowa : Humana Press, c2008. – (58.17/M592/v.387)

Contents

    Contents
    
    Preface
    Contributors
    PART 1 EXPERIMENTAL PROCEDURES
    1 SAGE and LongSAGE
    2 Robust-LongSAGE (RL-SAGE): An Improved LongSAGE Method for High-Throughput Transcriptome Analysis
    3 aRNA-LongSAGE: SAGE With Antisense RNA
    4 SuperSAGE
    5 Low-Cost-Medium Throughput Sanger Dideoxy Sequencing
    6 DeepSAGE: Higher Sensitivity and Multiplexing of Samples Using a Simpler Experimental Protocol
    7 High-Resolution， Genome-Wide Mapping of Chromatin Modifications by GMAT
    8 5'- and 3'-RACE from LongSAGE Tags
    PART 2 TAG EXTRACTION AND ANALYSIS
    9 Extraction and Annotation of SAGE Tags Using Sequence Quality Values
    10 Correction of Technology-Related Artifacts in Serial Analysis of Gene Expression
    11 Duplicate Ditag Analysis in LongSAGE
    12 Statistical Comparison of Two or More SAGE Libraries: One Tag at A Time
    13 Scaling of Gene Expression Data Allowing the Comparison of Different Gene Expression Platforms
    14 Clustering Analysis of SAGE Transcription Profiles Using a Poisson Approach
    15 Identifying Nonspecific SAGE Tags by Context of Gene Expression