Environmental genomics / edited by C. Cristofre Martin. — Totowa : Humana Press, c2008. – (58.17/M592/v.410)

Contents

    Contents
    
    Preface
    Contributors
    PART I: GENE EXPRESSION PROFILING
    1. High-Throughput Whole Mount In Situ Hybridization of Zebrafish Embryos for Analysis of Tissue-Specific Gene Expression Changes After Environmental Perturbation
    2. Fluorescent RNA Arbitrarily Primed Polymerase Chain Reaction
    3. Isolation of O3-Response Genes from Arabidopsis thaliana Using cDNA Macroarray
    4. Use of cDNA Macroarrays and Gene Profiling for Detection of Effects of Environmental Toxicants
    5. Constructing and Screening a cDNA Library
    6. Comparative Molecular Physiological Genomics
    7. Proteomic Analysis of Neuroendocrine Peptidergic System Disruption Using the AtT20 Pituitary Cell Line as a Model
    8. Proteomics-Based Method for Risk Assessment of Peroxisome Proliferating Pollutants in the Marine Environment
    9. Environmental Metabolomics Using 1H-NMR Spectroscopy
    PART II: DETECTION OF WHOLE GENOME MUTATION
    10. Restriction Landmark Genome Scanning for the Detection of Mutations
    11. Use of the Comet Assay in Environmental Toxicology
    12. The Micronucleus Assay Determination of Chromosomal Level DNA Damage
    13. Fluorescence In Situ Hybridization for the Detection of Chromosome Aberrations and Aneuploidy induced by Environmental Toxicants
    14. Laboratory Methods for the Detection of Chromosomal Structural Aberrations in Human and Mouse Sperm by Fluorescence In Situ Hybridization
    PART II1: DETERMINATION OF SPECIES DIVERSITY
    15. Assembling DNA Barcodes
    16. Application of Suppressive Subtractive Hybridization to Uncover the Metagenomic Diversity of Environmental Samples
    17. 16S rRNA Targeted DGGE Fingerprinting of Microbial Communities
    18. An Emulsion Polymerase Chain Reaction-Based Method for Molecular Haplotyping
    Index 363