Environmental genomics / edited by C. Cristofre Martin. — Totowa : Humana Press, c2008. – (58.17/M592/v.410) |
Contents
Contents
Preface
Contributors
PART I: GENE EXPRESSION PROFILING
1. High-Throughput Whole Mount In Situ Hybridization of Zebrafish Embryos for Analysis of Tissue-Specific Gene Expression Changes After Environmental Perturbation
2. Fluorescent RNA Arbitrarily Primed Polymerase Chain Reaction
3. Isolation of O3-Response Genes from Arabidopsis thaliana Using cDNA Macroarray
4. Use of cDNA Macroarrays and Gene Profiling for Detection of Effects of Environmental Toxicants
5. Constructing and Screening a cDNA Library
6. Comparative Molecular Physiological Genomics
7. Proteomic Analysis of Neuroendocrine Peptidergic System Disruption Using the AtT20 Pituitary Cell Line as a Model
8. Proteomics-Based Method for Risk Assessment of Peroxisome Proliferating Pollutants in the Marine Environment
9. Environmental Metabolomics Using 1H-NMR Spectroscopy
PART II: DETECTION OF WHOLE GENOME MUTATION
10. Restriction Landmark Genome Scanning for the Detection of Mutations
11. Use of the Comet Assay in Environmental Toxicology
12. The Micronucleus Assay Determination of Chromosomal Level DNA Damage
13. Fluorescence In Situ Hybridization for the Detection of Chromosome Aberrations and Aneuploidy induced by Environmental Toxicants
14. Laboratory Methods for the Detection of Chromosomal Structural Aberrations in Human and Mouse Sperm by Fluorescence In Situ Hybridization
PART II1: DETERMINATION OF SPECIES DIVERSITY
15. Assembling DNA Barcodes
16. Application of Suppressive Subtractive Hybridization to Uncover the Metagenomic Diversity of Environmental Samples
17. 16S rRNA Targeted DGGE Fingerprinting of Microbial Communities
18. An Emulsion Polymerase Chain Reaction-Based Method for Molecular Haplotyping
Index 363