Molecular motors : methods and protocols / edited by Ann O. Sperry. — Totowa : Humana Press, c2007. – (58.17/M592/v.392)

Contents

    Contents
    
    Preface
    Contributors
    1 Equilibrium Binding of Proteins to F-Actin
    2 Analysis of Calcium/Calmodulin Regulation of a Plant Kinesin Using Co-Sedimentation and ATPase Assays
    3 In Vitro and In Vivo Analysis of Microtubule-Destabilizing Kinesins
    4 Approaches to Kinesin-1 Phosphorylation
    5 Protein Modification to Probe Intradynein Interactions and In Vivo Redox State
    6 Methods to Study the Interactions of the Dynein Light Chains and Intermediate Chains
    7 Identification of Motor Protein Cargo by Yeast 2-Hybrid and Affinity Approaches
    8 In Situ Binding Assay to Detect Myosin-lc Interactions with Hair-Cell Proteins
    9 UItrastructural Analysis of Kinesin-Related Motor Proteins During Spermatogenesis
    10 In Vitro Motility System to Study the Role of Motor Proteins in Receptor-Ligand Sorting
    11 Enrichment and Disassembly of Ectoplasmic Specializations in the Rat Testis
    12 Single-Molecule Observation of Rotation of F1-ATPase Through Microbeads
    13 The Use of FRET in the Analysis of Motor Protein Structure
    14 Structure Determination of the Motor Domain of Yeast Kinesin Kar3 by X-Ray Crystallography
    15 High-Resolution Structural Analysis of the Kinesin-Microtubule Complex by Electron Cryo-Microscopy
    16 Chemical-Genetic Inhibition of Sensitized Mutant Unconventional Myosins
    Index